CATH: increased structural coverage of functional space #MMPMID33237325
Sillitoe I; Bordin N; Dawson N; Waman VP; Ashford P; Scholes HM; Pang CSM; Woodridge L; Rauer C; Sen N; Abbasian M; Le Cornu S; Lam SD; Berka K; Varekova IH; Svobodova R; Lees J; Orengo CA
Nucleic Acids Res 2021[Jan]; 49 (D1): D266-D273 PMID33237325show ga
CATH (https://www.cathdb.info) identifies domains in protein structures from wwPDB and classifies these into evolutionary superfamilies, thereby providing structural and functional annotations. There are two levels: CATH-B, a daily snapshot of the latest domain structures and superfamily assignments, and CATH+, with additional derived data, such as predicted sequence domains, and functionally coherent sequence subsets (Functional Families or FunFams). The latest CATH+ release, version 4.3, significantly increases coverage of structural and sequence data, with an addition of 65,351 fully-classified domains structures (+15%), providing 500 238 structural domains, and 151 million predicted sequence domains (+59%) assigned to 5481 superfamilies. The FunFam generation pipeline has been re-engineered to cope with the increased influx of data. Three times more sequences are captured in FunFams, with a concomitant increase in functional purity, information content and structural coverage. FunFam expansion increases the structural annotations provided for experimental GO terms (+59%). We also present CATH-FunVar web-pages displaying variations in protein sequences and their proximity to known or predicted functional sites. We present two case studies (1) putative cancer drivers and (2) SARS-CoV-2 proteins. Finally, we have improved links to and from CATH including SCOP, InterPro, Aquaria and 2DProt.
free
free
free
Nucleic+Acids+Res 2021 ; 49 (D1): D266-D273
