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10.2144/btn-2020-0133

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33222514!7888512!33222514
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suck abstract from ncbi


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pmid33222514      Biotechniques 2021 ; 70 (1): 7-20
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  • Increased sensitivity using real-time dPCR for detection of SARS-CoV-2 #MMPMID33222514
  • Duong K; Ou J; Li Z; Lv Z; Dong H; Hu T; Zhang Y; Hanna A; Gordon S; Crynen G; Head SR; Ordoukhanian P; Wang Y
  • Biotechniques 2021[Jan]; 70 (1): 7-20 PMID33222514show ga
  • A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end point dPCR compared with real-time qPCR, and the limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals. Real-time dPCR showed increased linear dynamic range compared with end point dPCR based on quantitation from amplification curves. Real-time dPCR can improve the performance of TaqMan assays beyond real-time qPCR and end point dPCR with better sensitivity and specificity, absolute quantification and a wider linear range of detection.
  • |*Real-Time Polymerase Chain Reaction[MESH]
  • |COVID-19 Nucleic Acid Testing/*methods/statistics & numerical data[MESH]
  • |COVID-19/diagnosis[MESH]
  • |Endpoint Determination[MESH]
  • |Humans[MESH]


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