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10.1016/j.virol.2020.11.002 http://scihub22266oqcxt.onion/10.1016/j.virol.2020.11.002 33220618!7664480!33220618     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2021 ; 553 (ä): 35-45 Nephropedia Template TP {{tp|p=33220618|t=2021. Development and utilization of an infectious clone for porcine deltacoronavirus strain USA/IL/2014/026 |pdf=|usr=}}{{33220618}} gab.com Text Development and utilization of an infectious clone for porcine deltacoronavirus strain USA/IL/2014/026 JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=33220618 #FOAvip We report the generation of a full-length infectious cDNA clone for porcine deltacoronavirus strain USA/IL/2014/026. Similar to the parental strain, the infectious clone virus (icPDCoV) replicated efficiently in cell culture and caused mild clinical symptoms in piglets. To investigate putative viral interferon (IFN) antagonists, we generated two mutant viruses: a nonstructural protein 15 mutant virus that encodes a catalytically-inactive endoribonuclease (icEnUmut), and an accessory gene NS6-deletion virus in which the NS6 gene was replaced with the mNeonGreen sequence (icDelNS6/nG). By infecting PK1 cells with these recombinant PDCoVs, we found that icDelNS6/nG elicited similar levels of type I IFN responses as icPDCoV, however icEnUmut stimulated robust type I IFN responses, demonstrating that the deltacoronavirus endoribonuclease, but not NS6, functions as an IFN antagonist in PK1 cells. Collectively, the construction of a full-length infectious clone and the identification of an IFN-antagonistic endoribonuclease will aid in the development of live-attenuated deltacoronavirus vaccines. Twit Text FOAVip Development and utilization of an infectious clone for porcine deltacoronavirus strain USA/IL/2014/026 ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=33220618 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33220618 #FOAvip English Wikipedia <ref>{{Cite pmid|33220618}}</ref> Development and utilization of an infectious clone for porcine deltacoronavirus strain USA/IL/2014/026 #MMPMID33220618 Deng X; Buckley AC; Pillatzki A; Lager KM; Baker SC; Faaberg KS Virology 2021[Jan]; 553 (ä): 35-45 PMID33220618show ga We report the generation of a full-length infectious cDNA clone for porcine deltacoronavirus strain USA/IL/2014/026. Similar to the parental strain, the infectious clone virus (icPDCoV) replicated efficiently in cell culture and caused mild clinical symptoms in piglets. To investigate putative viral interferon (IFN) antagonists, we generated two mutant viruses: a nonstructural protein 15 mutant virus that encodes a catalytically-inactive endoribonuclease (icEnUmut), and an accessory gene NS6-deletion virus in which the NS6 gene was replaced with the mNeonGreen sequence (icDelNS6/nG). By infecting PK1 cells with these recombinant PDCoVs, we found that icDelNS6/nG elicited similar levels of type I IFN responses as icPDCoV, however icEnUmut stimulated robust type I IFN responses, demonstrating that the deltacoronavirus endoribonuclease, but not NS6, functions as an IFN antagonist in PK1 cells. Collectively, the construction of a full-length infectious clone and the identification of an IFN-antagonistic endoribonuclease will aid in the development of live-attenuated deltacoronavirus vaccines. |Animals[MESH] |Clone Cells[MESH] |Coronavirus Infections/pathology[MESH] |DNA, Complementary/*isolation & purification[MESH] |Deltacoronavirus/*genetics/pathogenicity/physiology[MESH] |Endoribonucleases/physiology[MESH] |Interferons/antagonists & inhibitors[MESH] |Swine/*virology[MESH]Development and utilization of an infectious clone for porcine deltaco(epmc).pdf DeepDyve Pubget Overpricing