Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.ijid.2020.11.155 http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.11.155 33220439!7674967!33220439     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Infect+Dis 2021 ; 103 (ä): 19-22 Nephropedia Template TP {{tp|p=33220439|t=2021. Increasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling |pdf=|usr=}}{{33220439}} gab.com Text Increasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33220439 #FOAvip OBJECTIVES: Limited testing capacity has characterized the ongoing coronavirus disease 2019 (COVID-19) pandemic in Spain, hampering timely control of outbreaks and opportunities to reduce the escalation of community transmission. This study investigated the potential to use sample pooling, followed by one-step retrotranscription and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to increase testing capacity for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). METHODS: Various pool sizes (five, 10 and 15 samples) were evaluated prior to RNA extraction followed by standard RT-qPCR for the diagnosis of COVID-19. The pool size achieving reproducible results with individual sample testing was subsequently used to assess nasopharyngeal samples in a tertiary hospital in August 2020. RESULTS: A pool size of five samples had higher sensitivity compared with pool sizes of 10 and 15 samples, showing a mean cycle threshold (Ct) shift of 3.5 [standard deviation (SD) 2.2] between the pooled test and positive samples in the pool. Next, a pool size of five was used to test a total of 895 pools (4475 prospective samples) using two different RT-qPCR kits. The Real Accurate Quadruplex corona-plus PCR Kit (PathoFinder) reported the lowest mean Ct shift [2.2 (SD 2.4)] between the pool and individual samples. This strategy enables detection of individual positive samples in positive pools with Ct of 16.7-39.4. CONCLUSIONS: Grouping samples into pools of five for RT-qPCR resulted in an increase in SARS-CoV-2 testing capacity with minimal loss of sensitivity compared with testing each sample individually. Twit Text FOAVip Increasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33220439 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33220439 #FOAvip English Wikipedia <ref>{{Cite pmid|33220439}}</ref> Increasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling #MMPMID33220439 Alcoba-Florez J; Gil-Campesino H; Garcia-Martinez de Artola D; Diez-Gil O; Valenzuela-Fernandez A; Gonzalez-Montelongo R; Ciuffreda L; Flores C Int J Infect Dis 2021[Feb]; 103 (ä): 19-22 PMID33220439show ga OBJECTIVES: Limited testing capacity has characterized the ongoing coronavirus disease 2019 (COVID-19) pandemic in Spain, hampering timely control of outbreaks and opportunities to reduce the escalation of community transmission. This study investigated the potential to use sample pooling, followed by one-step retrotranscription and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to increase testing capacity for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). METHODS: Various pool sizes (five, 10 and 15 samples) were evaluated prior to RNA extraction followed by standard RT-qPCR for the diagnosis of COVID-19. The pool size achieving reproducible results with individual sample testing was subsequently used to assess nasopharyngeal samples in a tertiary hospital in August 2020. RESULTS: A pool size of five samples had higher sensitivity compared with pool sizes of 10 and 15 samples, showing a mean cycle threshold (Ct) shift of 3.5 [standard deviation (SD) 2.2] between the pooled test and positive samples in the pool. Next, a pool size of five was used to test a total of 895 pools (4475 prospective samples) using two different RT-qPCR kits. The Real Accurate Quadruplex corona-plus PCR Kit (PathoFinder) reported the lowest mean Ct shift [2.2 (SD 2.4)] between the pool and individual samples. This strategy enables detection of individual positive samples in positive pools with Ct of 16.7-39.4. CONCLUSIONS: Grouping samples into pools of five for RT-qPCR resulted in an increase in SARS-CoV-2 testing capacity with minimal loss of sensitivity compared with testing each sample individually. |*COVID-19 Testing[MESH] |*SARS-CoV-2[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Prospective Studies[MESH] |Reagent Kits, Diagnostic[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]Increasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling (epmc).pdf DeepDyve Pubget Overpricing