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10.1038/s41467-020-19258-y http://scihub22266oqcxt.onion/10.1038/s41467-020-19258-y 33219228!7679446!33219228     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2020 ; 11 (1): 5920 Nephropedia Template TP {{tp|p=33219228|t=2020. An enhanced isothermal amplification assay for viral detection |pdf=|usr=}}{{33219228}} gab.com Text An enhanced isothermal amplification assay for viral detection JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33219228 #FOAvip Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability. Twit Text FOAVip An enhanced isothermal amplification assay for viral detection ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33219228 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33219228 #FOAvip English Wikipedia <ref>{{Cite pmid|33219228}}</ref> An enhanced isothermal amplification assay for viral detection #MMPMID33219228 Qian J; Boswell SA; Chidley C; Lu ZX; Pettit ME; Gaudio BL; Fajnzylber JM; Ingram RT; Ward RH; Li JZ; Springer M Nat Commun 2020[Nov]; 11 (1): 5920 PMID33219228show ga Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability. |Betacoronavirus/*genetics/*isolation & purification[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques[MESH] |Coronavirus Infections/diagnosis[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |RNA, Viral/genetics/isolation & purification[MESH] |RNA-Directed DNA Polymerase/metabolism[MESH] |RNA/metabolism[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Recombinases/metabolism[MESH] |SARS-CoV-2[MESH] |Saliva/virology[MESH]An enhanced isothermal amplification assay for viral detection (epmc).pdf DeepDyve Pubget Overpricing