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10.1038/s41467-020-19097-x http://scihub22266oqcxt.onion/10.1038/s41467-020-19097-x 33219225!7680145!33219225     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2020 ; 11 (1): 5921 Nephropedia Template TP {{tp|p=33219225|t=2020. Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2 |pdf=|usr=}}{{33219225}} gab.com Text Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2 JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33219225 #FOAvip The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE's results can be visualized with an in-tube fluorescent readout - reducing contamination risk as amplification reaction tubes remain sealed - and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities. Twit Text FOAVip Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2 ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33219225 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33219225 #FOAvip English Wikipedia <ref>{{Cite pmid|33219225}}</ref> Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2 #MMPMID33219225 Arizti-Sanz J; Freije CA; Stanton AC; Petros BA; Boehm CK; Siddiqui S; Shaw BM; Adams G; Kosoko-Thoroddsen TF; Kemball ME; Uwanibe JN; Ajogbasile FV; Eromon PE; Gross R; Wronka L; Caviness K; Hensley LE; Bergman NH; MacInnis BL; Happi CT; Lemieux JE; Sabeti PC; Myhrvold C Nat Commun 2020[Nov]; 11 (1): 5921 PMID33219225show ga The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE's results can be visualized with an in-tube fluorescent readout - reducing contamination risk as amplification reaction tubes remain sealed - and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities. |Betacoronavirus/*isolation & purification[MESH] |Biological Assay[MESH] |COVID-19[MESH] |CRISPR-Associated Proteins/*metabolism[MESH] |Coronavirus Infections/diagnosis/virology[MESH] |Fluorescence[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/diagnosis/virology[MESH]Streamlined inactivation, amplification, and Cas13-based detection of (epmc).pdf DeepDyve Pubget Overpricing