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10.3390/jof6040293 http://scihub22266oqcxt.onion/10.3390/jof6040293 33217898!7711936!33217898     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33217898&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Fungi+(Basel) 2020 ; 6 (4): ? Nephropedia Template TP {{tp|p=33217898|t=2020. Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives |pdf=|usr=}}{{33217898}} gab.com Text Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives JO: J Fungi (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33217898 #FOAvip Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 degrees C and as budding yeasts when cultured at 37 degrees C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recent achievements in molecular diagnostics of paracoccidioidomycosis, including the main advantages and pitfalls related to each technique. We discuss these breakthroughs in light of taxonomic changes in the Paracoccidioides genus. Twit Text FOAVip Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives ????J Fungi (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33217898 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33217898 #FOAvip English Wikipedia <ref>{{Cite pmid|33217898}}</ref> Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives #MMPMID33217898 Pinheiro BG; Hahn RC; Camargo ZP; Rodrigues AM J Fungi (Basel) 2020[Nov]; 6 (4): ? PMID33217898show ga Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 degrees C and as budding yeasts when cultured at 37 degrees C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recent achievements in molecular diagnostics of paracoccidioidomycosis, including the main advantages and pitfalls related to each technique. We discuss these breakthroughs in light of taxonomic changes in the Paracoccidioides genus. ?Molecular Tools for Detection and Identification of Paracoccidioides S(epmc).pdf DeepDyve Pubget Overpricing