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10.1016/j.antiviral.2020.104974

http://scihub22266oqcxt.onion/10.1016/j.antiviral.2020.104974
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33217430!7670965!33217430
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suck abstract from ncbi


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pmid33217430      Antiviral+Res 2021 ; 185 (ä): 104974
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  • A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2 #MMPMID33217430
  • Zhang Y; Song W; Chen S; Yuan Z; Yi Z
  • Antiviral Res 2021[Jan]; 185 (ä): 104974 PMID33217430show ga
  • Vaccines and antiviral agents are in urgent need to stop the COVID-19 pandemic. To facilitate antiviral screening against SARS-CoV-2 without requirement for high biosafety level facility, we developed a bacterial artificial chromosome (BAC)-vectored replicon of SARS-CoV-2, nCoV-SH01 strain, in which secreted Gaussia luciferase (sGluc) was encoded in viral subgenomic mRNA as a reporter gene. The replicon was devoid of structural genes spike (S), membrane (M), and envelope (E). Upon transfection, the replicon RNA replicated in various cell lines, and was sensitive to interferon alpha (IFN-alpha), remdesivir, but was resistant to hepatitis C virus inhibitors daclatasvir and sofosbuvir. Replication of the replicon was also sensitive overexpression to zinc-finger antiviral protein (ZAP). We also constructed a four-plasmid in-vitro ligation system that is compatible with the BAC system, which makes it easy to introduce desired mutations into the assembly plasmids for in-vitro ligation. This replicon system would be helpful for performing antiviral screening and dissecting virus-host interactions.
  • |*Chromosomes, Artificial, Bacterial[MESH]
  • |Adenosine Monophosphate/analogs & derivatives/pharmacology[MESH]
  • |Alanine/analogs & derivatives/pharmacology[MESH]
  • |Animals[MESH]
  • |Antiviral Agents/*pharmacology[MESH]
  • |COVID-19 Drug Treatment[MESH]
  • |COVID-19/*virology[MESH]
  • |Cell Line[MESH]
  • |Chlorocebus aethiops[MESH]
  • |HEK293 Cells[MESH]
  • |Hepacivirus/drug effects[MESH]
  • |Hepatitis C/drug therapy/virology[MESH]
  • |Humans[MESH]
  • |Interferon-alpha/pharmacology[MESH]
  • |RNA-Binding Proteins/biosynthesis/genetics[MESH]
  • |Replicon/*drug effects[MESH]
  • |SARS-CoV-2/*drug effects/*genetics[MESH]
  • |Sofosbuvir/pharmacology[MESH]
  • |Vero Cells[MESH]


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  • suck abstract from ncbi

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