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10.1093/labmed/lmaa094 http://scihub22266oqcxt.onion/10.1093/labmed/lmaa094 33206195!7717336!33206195     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Lab+Med 2021 ; 52 (1): e15-e16 Nephropedia Template TP {{tp|p=33206195|t=2021. Impact of Pool Testing in Detection of Asymptomatic Patients with COVID-19 |pdf=|usr=}}{{33206195}} gab.com Text Impact of Pool Testing in Detection of Asymptomatic Patients with COVID-19 JO: Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=33206195 #FOAvip OBJECTIVE: During the current pandemic, COVID-19 has been detected in patients using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) that confirms the presence of SARS-CoV-2 RNA. The demand for increased testing, particularly for asymptomatic individuals required alternative approaches to single-patient RT-PCR testing, such as pooling. METHODS: This study explored the impact of dilution on the detectability of SARS-CoV-2 in asymptomatic patients using RT-PCR and demonstrated that pooling can be effective in low prevalence populations. RESULTS: The RT-PCR results for the 3:1, 5:1, and 7:1 aliquot samples showed little differences in CT values, confirming detection capability at these dilutions. CONCLUSION: Based on the results of the present study, a pooled approach with up to 5:1 sample aliquots and using the current RT-PCR methodology likely will detect SARS CoV2 RNA among asymptomatic patients. Twit Text FOAVip Impact of Pool Testing in Detection of Asymptomatic Patients with COVID-19 ????Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=33206195 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33206195 #FOAvip English Wikipedia <ref>{{Cite pmid|33206195}}</ref> Impact of Pool Testing in Detection of Asymptomatic Patients with COVID-19 #MMPMID33206195 Smalley DL; Cisarik PM; Grantham J; Cloud W; Neil RB; DePriest P Lab Med 2021[Jan]; 52 (1): e15-e16 PMID33206195show ga OBJECTIVE: During the current pandemic, COVID-19 has been detected in patients using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) that confirms the presence of SARS-CoV-2 RNA. The demand for increased testing, particularly for asymptomatic individuals required alternative approaches to single-patient RT-PCR testing, such as pooling. METHODS: This study explored the impact of dilution on the detectability of SARS-CoV-2 in asymptomatic patients using RT-PCR and demonstrated that pooling can be effective in low prevalence populations. RESULTS: The RT-PCR results for the 3:1, 5:1, and 7:1 aliquot samples showed little differences in CT values, confirming detection capability at these dilutions. CONCLUSION: Based on the results of the present study, a pooled approach with up to 5:1 sample aliquots and using the current RT-PCR methodology likely will detect SARS CoV2 RNA among asymptomatic patients. |Asymptomatic Diseases[MESH] |Buffers[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |RNA, Viral/*genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/*genetics[MESH]Impact of Pool Testing in Detection of Asymptomatic Patients with COVI(epmc).pdf DeepDyve Pubget Overpricing