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10.1080/22221751.2020.1852893

http://scihub22266oqcxt.onion/10.1080/22221751.2020.1852893
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suck abstract from ncbi


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pmid33206004      Emerg+Microbes+Infect 2020 ; 9 (1): 2547-2549
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  • A lateral flow immunoassay test performance in SARS-CoV-2 seroprevalence surveys: a validation study among healthcare workers #MMPMID33206004
  • Garlantezec R; Heslan C; Tadie E; Tattevin P; Thibault V; Paris C
  • Emerg Microbes Infect 2020[Dec]; 9 (1): 2547-2549 PMID33206004show ga
  • The objective of this study was to evaluate the validity and reliability of NG-Test(R) when used as a finger-prick test on healthcare workers and to compare it to the ELISA Wantai Immunoassay. Fifty-one healthcare workers who were RT-PCR SARS-CoV-2 positive and 59 who were RT-PCR SARS-CoV-2 negative accepted to participate in this study. They were subjected to an NG-Test(R) finger-prick test and collection of a blood sample on the same day. A second NG-Test(R) on another finger was performed for the first 30 cases and controls and read blinded to the first. Sera obtained from blood samples were used to perform the Wantai SARS-CoV-2 ELISA. The interobserver agreement for the NG-Test(R) test was perfect (kappa coefficient = 100% [98%-100%]). The sensitivity of NG-Test(R) was estimated to be 85% [71.9%-92.3%] and the specificity 98.3% [95.0%-100.0%]) for both IgG and IgM. The percentage of agreement between the Wantai immunoassay and NG-Test(R) was 92.73% for IgG (Kappa = 0.85 [0.75-0.95]) and 65.45% (Kappa = 0.42 [0.26-0.58]) for IgM. Our study highlights the need to validate rapid immunoassay tests under real-life conditions. If NG-Test(R) is used in seroprevalence surveys, we recommend that its diagnostic performance be taken into consideration to obtain a reliable estimation.
  • |Adult[MESH]
  • |Antibodies, Viral/*analysis[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Female[MESH]
  • |Health Personnel/*statistics & numerical data[MESH]
  • |Humans[MESH]
  • |Immunoassay/*standards[MESH]
  • |Immunoglobulin A/analysis[MESH]
  • |Immunoglobulin G/analysis[MESH]
  • |Immunoglobulin M/analysis[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Reagent Kits, Diagnostic/*standards[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Seroepidemiologic Studies[MESH]
  • |Serologic Tests/*standards[MESH]


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