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10.1021/acsinfecdis.0c00664 http://scihub22266oqcxt.onion/10.1021/acsinfecdis.0c00664 33205948!ä!33205948 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Infect+Dis 2020 ; 6 (12): 3269-3276 Nephropedia Template TP {{tp|p=33205948|t=2020. Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with High Resolution Mass Spectrometry |pdf=|usr=}}{{33205948}} gab.com Text Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with High Resolution Mass Spectrometry JO: ACS Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33205948 #FOAvip A high resolution mass spectrometry approach has been applied for the first time to detect and characterize SARS-CoV-2 coronavirus in cell cultured and nasopharyngeal swab specimens. Peptide ions for three of the most abundant structural viral proteins (membrane, nucleocapid, and spike) are detected and assigned directly, by virtue of the high resolution and mass accuracy within the mass maps of whole virus digests, without the need for tandem mass spectrometry (MS/MS). MALDI-MS based approaches offer high sample throughput and speed, compared with those of LC-MS strategies, and detection limits at some 10(5) copies, or orders of magnitude less with selected ion monitoring, that compete favorably with conventional reverse transcription polymerase chain reaction (RT-PCR) strategies. The detection of signature peptides unique to SARS-CoV-2 coronavirus over those from the influenza virus allows for its unambiguous detection. Twit Text FOAVip Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with High Resolution Mass Spectrometry ????ACS Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33205948 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33205948 #FOAvip English Wikipedia <ref>{{Cite pmid|33205948}}</ref> Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with High Resolution Mass Spectrometry #MMPMID33205948 Dollman NL; Griffin JH; Downard KM ACS Infect Dis 2020[Dec]; 6 (12): 3269-3276 PMID33205948show ga A high resolution mass spectrometry approach has been applied for the first time to detect and characterize SARS-CoV-2 coronavirus in cell cultured and nasopharyngeal swab specimens. Peptide ions for three of the most abundant structural viral proteins (membrane, nucleocapid, and spike) are detected and assigned directly, by virtue of the high resolution and mass accuracy within the mass maps of whole virus digests, without the need for tandem mass spectrometry (MS/MS). MALDI-MS based approaches offer high sample throughput and speed, compared with those of LC-MS strategies, and detection limits at some 10(5) copies, or orders of magnitude less with selected ion monitoring, that compete favorably with conventional reverse transcription polymerase chain reaction (RT-PCR) strategies. The detection of signature peptides unique to SARS-CoV-2 coronavirus over those from the influenza virus allows for its unambiguous detection. |COVID-19/*diagnosis/virology[MESH] |Coronavirus Nucleocapsid Proteins/*chemistry[MESH] |Humans[MESH] |Peptide Mapping/*methods[MESH] |Phosphoproteins/chemistry[MESH] |Proteolysis[MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods[MESH] |SARS-CoV-2/*chemistry/genetics[MESH] |Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods[MESH] |Spike Glycoprotein, Coronavirus/*chemistry[MESH]Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with Hi(epmc).pdf DeepDyve Pubget Overpricing