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10.1186/s13059-020-02193-y http://scihub22266oqcxt.onion/10.1186/s13059-020-02193-y 33203452!7670108!33203452     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Biol 2020 ; 21 (1): 279 Nephropedia Template TP {{tp|p=33203452|t=2020. A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila |pdf=|usr=}}{{33203452}} gab.com Text A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=33203452 #FOAvip Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 different Cas13 variants to identify enzymes with the best overall performance and show that Cas13 could target endogenous Drosophila transcripts in vivo with high efficiency and specificity. We also develop Cas13 applications to edit mRNAs and target mitochondrial transcripts. Our vector collection represents a versatile tool collection to manipulate gene expression at the post-transcriptional level. Twit Text FOAVip A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=33203452 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33203452 #FOAvip English Wikipedia <ref>{{Cite pmid|33203452}}</ref> A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila #MMPMID33203452 Huynh N; Depner N; Larson R; King-Jones K Genome Biol 2020[Nov]; 21 (1): 279 PMID33203452show ga Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 different Cas13 variants to identify enzymes with the best overall performance and show that Cas13 could target endogenous Drosophila transcripts in vivo with high efficiency and specificity. We also develop Cas13 applications to edit mRNAs and target mitochondrial transcripts. Our vector collection represents a versatile tool collection to manipulate gene expression at the post-transcriptional level. |*CRISPR-Cas Systems[MESH] |*RNA Processing, Post-Transcriptional[MESH] |Adenosine Deaminase/metabolism[MESH] |Animals[MESH] |CRISPR-Associated Proteins/metabolism[MESH] |Drosophila/*genetics[MESH] |Endonucleases/metabolism[MESH] |Gene Expression[MESH] |RNA, Mitochondrial[MESH] |RNA-Binding Proteins/metabolism[MESH]A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosoph(epmc).pdf DeepDyve Pubget Overpricing