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10.3389/fcimb.2020.582187 http://scihub22266oqcxt.onion/10.3389/fcimb.2020.582187 33194818!7642909!33194818     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Cell+Infect+Microbiol 2020 ; 10 (ä): 582187 Nephropedia Template TP {{tp|p=33194818|t=2020. Virome Sequencing of the Human Intestinal Mucosal-Luminal Interface |pdf=|usr=}}{{33194818}} gab.com Text Virome Sequencing of the Human Intestinal Mucosal-Luminal Interface JO: Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33194818 #FOAvip While the human gut virome has been increasingly explored in recent years, nearly all studies have been limited to fecal sampling. The mucosal-luminal interface has been established as a viable sample type for profiling the microbial biogeography of the gastrointestinal tract. We have developed a protocol to extract nucleic acids from viruses at the mucosal-luminal interface of the proximal and distal colon. Colonic viromes from pediatric patients with Crohn's disease demonstrated high interpatient diversity and low but significant intrapatient variation between sites. Whole metagenomics was also performed to explore virome-bacteriome interactions and to compare the viral communities observed in virome and whole metagenomic sequencing. A site-specific study of the human gut virome is a necessary step to advance our understanding of virome-bacteriome-host interactions in human diseases. Twit Text FOAVip Virome Sequencing of the Human Intestinal Mucosal-Luminal Interface ????Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33194818 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33194818 #FOAvip English Wikipedia <ref>{{Cite pmid|33194818}}</ref> Virome Sequencing of the Human Intestinal Mucosal-Luminal Interface #MMPMID33194818 Yan A; Butcher J; Mack D; Stintzi A Front Cell Infect Microbiol 2020[]; 10 (ä): 582187 PMID33194818show ga While the human gut virome has been increasingly explored in recent years, nearly all studies have been limited to fecal sampling. The mucosal-luminal interface has been established as a viable sample type for profiling the microbial biogeography of the gastrointestinal tract. We have developed a protocol to extract nucleic acids from viruses at the mucosal-luminal interface of the proximal and distal colon. Colonic viromes from pediatric patients with Crohn's disease demonstrated high interpatient diversity and low but significant intrapatient variation between sites. Whole metagenomics was also performed to explore virome-bacteriome interactions and to compare the viral communities observed in virome and whole metagenomic sequencing. A site-specific study of the human gut virome is a necessary step to advance our understanding of virome-bacteriome-host interactions in human diseases. |*Gastrointestinal Microbiome[MESH] |*Viruses/genetics[MESH] |Child[MESH] |Feces[MESH] |Humans[MESH] |Metagenome[MESH] |Metagenomics[MESH]Virome Sequencing of the Human Intestinal Mucosal-Luminal Interface (epmc).pdf DeepDyve Pubget Overpricing