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10.1007/s10096-020-04102-4 http://scihub22266oqcxt.onion/10.1007/s10096-020-04102-4 33184753!7661101!33184753     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33184753&cmd=llinks): Failed to open stream: HTTP request failed! 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Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteins |pdf=|usr=}}{{33184753}} gab.com Text Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteins JO: Eur J Clin Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33184753 #FOAvip Serological test is a valuable diagnostic tool for coronavirus disease 2019 (COVID-19). However, considerable improvements to these tests are needed, especially in the detection sensitivity. In this study, six recombinant nucleocapsid and spike proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were prepared and evaluated, including three prokaryotic expression nucleocapsid proteins (rN, rN1, rN2) and three eukaryotic expression spike proteins (rS1, rS-RBD, rS-RBD-mFc). The recombinant proteins with the highest ELISA titers (rS1 and rS-RBD-mFc) were selected to develop a double-antigen sandwich colloidal gold immunochromatography assay (GICA) to detect total antibodies against SARS-CoV-2. The clinical evaluation results showed that the sensitivity and specificity of GICA were 92.09% (419/455) and 99.44% (706/710), respectively. Moreover, a significant number (65.63%, 21/32) of COVID-19 patients with undetectable viral RNA were correctly diagnosed by the GICA method. In conclusion, the eukaryotic expression spike proteins (rS1 and rS-RBD-mFc) are more suitable than the prokaryotic expression nucleocapsid proteins for serological diagnosis of SARS-CoV-2. The proposed GICA for detection of total antibodies could be a powerful complement to the current RNA tests for COVID-19. Twit Text FOAVip Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteins ????Eur J Clin Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33184753 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33184753 #FOAvip English Wikipedia <ref>{{Cite pmid|33184753}}</ref> Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteins #MMPMID33184753 Zhang P; Gao Q; Wang T; Ke Y; Mo F; Jia R; Liu W; Liu L; Zheng S; Liu Y; Li L; Wang Y; Xu L; Hao K; Min W; Liu X; Yang R; Li S; Lin C; Zhao Y Eur J Clin Microbiol Infect Dis 2021[May]; 40 (5): 921-928 PMID33184753show ga Serological test is a valuable diagnostic tool for coronavirus disease 2019 (COVID-19). However, considerable improvements to these tests are needed, especially in the detection sensitivity. In this study, six recombinant nucleocapsid and spike proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were prepared and evaluated, including three prokaryotic expression nucleocapsid proteins (rN, rN1, rN2) and three eukaryotic expression spike proteins (rS1, rS-RBD, rS-RBD-mFc). The recombinant proteins with the highest ELISA titers (rS1 and rS-RBD-mFc) were selected to develop a double-antigen sandwich colloidal gold immunochromatography assay (GICA) to detect total antibodies against SARS-CoV-2. The clinical evaluation results showed that the sensitivity and specificity of GICA were 92.09% (419/455) and 99.44% (706/710), respectively. Moreover, a significant number (65.63%, 21/32) of COVID-19 patients with undetectable viral RNA were correctly diagnosed by the GICA method. In conclusion, the eukaryotic expression spike proteins (rS1 and rS-RBD-mFc) are more suitable than the prokaryotic expression nucleocapsid proteins for serological diagnosis of SARS-CoV-2. The proposed GICA for detection of total antibodies could be a powerful complement to the current RNA tests for COVID-19. |Antibodies, Viral/blood[MESH] |COVID-19 Nucleic Acid Testing[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/blood/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins/genetics/immunology[MESH] |Humans[MESH] |Immunoassay[MESH] |Phosphoproteins/genetics/immunology[MESH] |RNA, Viral/genetics[MESH] |Recombinant Proteins/genetics/immunology[MESH] |SARS-CoV-2/genetics/immunology/*isolation & purification[MESH] |Sensitivity and Specificity[MESH]Development and evaluation of a serological test for diagnosis of COVI(epmc).pdf DeepDyve Pubget Overpricing