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Characterization of pre-existing and induced SARS-CoV-2-specific CD8(+) T cells #MMPMID33184509
Schulien I; Kemming J; Oberhardt V; Wild K; Seidel LM; Killmer S; Sagar; Daul F; Salvat Lago M; Decker A; Luxenburger H; Binder B; Bettinger D; Sogukpinar O; Rieg S; Panning M; Huzly D; Schwemmle M; Kochs G; Waller CF; Nieters A; Duerschmied D; Emmerich F; Mei HE; Schulz AR; Llewellyn-Lacey S; Price DA; Boettler T; Bengsch B; Thimme R; Hofmann M; Neumann-Haefelin C
Nat Med 2021[Jan]; 27 (1): 78-85 PMID33184509show ga
Emerging data indicate that SARS-CoV-2-specific CD8(+) T cells targeting different viral proteins are detectable in up to 70% of convalescent individuals(1-5). However, very little information is currently available about the abundance, phenotype, functional capacity and fate of pre-existing and induced SARS-CoV-2-specific CD8(+) T cell responses during the natural course of SARS-CoV-2 infection. Here, we define a set of optimal and dominant SARS-CoV-2-specific CD8(+) T cell epitopes. We also perform a high-resolution ex vivo analysis of pre-existing and induced SARS-CoV-2-specific CD8(+) T cells, applying peptide-loaded major histocompatibility complex class I (pMHCI) tetramer technology. We observe rapid induction, prolonged contraction and emergence of heterogeneous and functionally competent cross-reactive and induced memory CD8(+) T cell responses in cross-sectionally analyzed individuals with mild disease following SARS-CoV-2 infection and three individuals longitudinally assessed for their T cells pre- and post-SARS-CoV-2 infection. SARS-CoV-2-specific memory CD8(+) T cells exhibited functional characteristics comparable to influenza-specific CD8(+) T cells and were detectable in SARS-CoV-2 convalescent individuals who were seronegative for anti-SARS-CoV-2 antibodies targeting spike (S) and nucleoprotein (N). These results define cross-reactive and induced SARS-CoV-2-specific CD8(+) T cell responses as potentially important determinants of immune protection in mild SARS-CoV-2 infection.
Nat+Med 2021 ; 27 (1): 78-85
