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10.1016/j.jiac.2020.10.026 http://scihub22266oqcxt.onion/10.1016/j.jiac.2020.10.026 33183963!7598429!33183963     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33183963&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Infect+Chemother 2021 ; 27 (2): 406-409 Nephropedia Template TP {{tp|p=33183963|t=2021. Analysis of a persistent viral shedding patient infected with SARS-CoV-2 by RT-qPCR, FilmArray Respiratory Panel v2 1, and antigen detection |pdf=|usr=}}{{33183963}} gab.com Text Analysis of a persistent viral shedding patient infected with SARS-CoV-2 by RT-qPCR, FilmArray Respiratory Panel v2 1, and antigen detection JO: J Infect Chemother http://www.kidney.de/pget.php?&tw=1&pmid=33183963 #FOAvip Various diagnostic tests utilizing different principles are currently under development for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, these tests can occasionally produce discrepant results, causing confusion in their interpretation. Here, we evaluated the performance and features of three diagnostic assays: quantitative reverse transcription polymerase chain reaction (RT-qPCR), FilmArray Respiratory Panel (RP) v2.1, and the LUMIPULSE antigen test. Twenty-seven serial nasopharyngeal swabs were collected from a prolonged viral shedding patient who had been hospitalized for 51 days. We examined the SARS-CoV-2 detection rates of the three tests. The overall agreement rate was 81% between RT-qPCR and FilmArray RP v2.1, 63% between the antigen test and FilmArray RP v2.1, and 59% between the antigen test and RT-qPCR. We obtained concordant results in samples with high viral loads (low threshold cycle values) by all three tests. RT-qPCR and FilmArray RP v2.1 accurately detected SARS-CoV-2 at the early to intermediate phases of infection, but the results varied at the late phase. The antigen test also produced a positive result at the early phase but varied at the intermediate phase and consistently produced negative results at late phase of infection. These results demonstrated FilmArray RP v2.1 could detect SARS-CoV-2 with accuracy comparable to RT-qPCR. Further, there were discrepant results using different types of diagnostic tests during the clinical course of prolonged viral shedding patient. We provided insights into how to utilize different types of kits to assess and manage SARS-CoV-2 infections. Twit Text FOAVip Analysis of a persistent viral shedding patient infected with SARS-CoV-2 by RT-qPCR, FilmArray Respiratory Panel v2 1, and antigen detection ????J Infect Chemother http://www.kidney.de/pget.php?&tw=1&pmid=33183963 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33183963 #FOAvip English Wikipedia <ref>{{Cite pmid|33183963}}</ref> Analysis of a persistent viral shedding patient infected with SARS-CoV-2 by RT-qPCR, FilmArray Respiratory Panel v2 1, and antigen detection #MMPMID33183963 Hirotsu Y; Maejima M; Shibusawa M; Amemiya K; Nagakubo Y; Hosaka K; Sueki H; Hayakawa M; Mochizuki H; Tsutsui T; Kakizaki Y; Miyashita Y; Omata M J Infect Chemother 2021[Feb]; 27 (2): 406-409 PMID33183963show ga Various diagnostic tests utilizing different principles are currently under development for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, these tests can occasionally produce discrepant results, causing confusion in their interpretation. Here, we evaluated the performance and features of three diagnostic assays: quantitative reverse transcription polymerase chain reaction (RT-qPCR), FilmArray Respiratory Panel (RP) v2.1, and the LUMIPULSE antigen test. Twenty-seven serial nasopharyngeal swabs were collected from a prolonged viral shedding patient who had been hospitalized for 51 days. We examined the SARS-CoV-2 detection rates of the three tests. The overall agreement rate was 81% between RT-qPCR and FilmArray RP v2.1, 63% between the antigen test and FilmArray RP v2.1, and 59% between the antigen test and RT-qPCR. We obtained concordant results in samples with high viral loads (low threshold cycle values) by all three tests. RT-qPCR and FilmArray RP v2.1 accurately detected SARS-CoV-2 at the early to intermediate phases of infection, but the results varied at the late phase. The antigen test also produced a positive result at the early phase but varied at the intermediate phase and consistently produced negative results at late phase of infection. These results demonstrated FilmArray RP v2.1 could detect SARS-CoV-2 with accuracy comparable to RT-qPCR. Further, there were discrepant results using different types of diagnostic tests during the clinical course of prolonged viral shedding patient. We provided insights into how to utilize different types of kits to assess and manage SARS-CoV-2 infections. |*Virus Shedding[MESH] |Antigens, Viral/analysis[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |Multiplex Polymerase Chain Reaction/methods[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/analysis[MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods[MESH] |SARS-CoV-2/immunology/*isolation & purification[MESH] |Sensitivity and Specificity[MESH]Analysis of a persistent viral shedding patient infected with SARS-CoV(epmc).pdf DeepDyve Pubget Overpricing