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10.7554/eLife.57681 http://scihub22266oqcxt.onion/10.7554/eLife.57681 33179596!7707824!33179596     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2020 ; 9 (ä): ä Nephropedia Template TP {{tp|p=33179596|t=2020. A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics |pdf=|usr=}}{{33179596}} gab.com Text A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=33179596 #FOAvip We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of lattice light-sheet microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections. Twit Text FOAVip A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=33179596 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33179596 #FOAvip English Wikipedia <ref>{{Cite pmid|33179596}}</ref> A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics #MMPMID33179596 Sapoznik E; Chang BJ; Huh J; Ju RJ; Azarova EV; Pohlkamp T; Welf ES; Broadbent D; Carisey AF; Stehbens SJ; Lee KM; Marin A; Hanker AB; Schmidt JC; Arteaga CL; Yang B; Kobayashi Y; Tata PR; Kruithoff R; Doubrovinski K; Shepherd DP; Millett-Sikking A; York AG; Dean KM; Fiolka RP Elife 2020[Nov]; 9 (ä): ä PMID33179596show ga We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of lattice light-sheet microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections. |Animals[MESH] |Cells, Cultured[MESH] |Humans[MESH] |Mice[MESH] |Microfluidic Analytical Techniques/instrumentation[MESH] |Microscopy, Confocal/*instrumentation/*methods[MESH] |Plasmids[MESH] |Rats[MESH]A versatile oblique plane microscope for large-scale and high-resoluti(epmc).pdf DeepDyve Pubget Overpricing