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10.2217/fmb-2020-0094 http://scihub22266oqcxt.onion/10.2217/fmb-2020-0094 33179525!ä!33179525 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Future+Microbiol 2020 ; ä (ä): 1483-1487 Nephropedia Template TP {{tp|p=33179525|t=2020. Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints |pdf=|usr=}}{{33179525}} gab.com Text Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints JO: Future Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33179525 #FOAvip RT-PCR-based assays for the detection of SARS-CoV-2 have played an essential role in the current COVID-19 pandemic. However, the sample collection and test reagents are in short supply, primarily due to supply chain issues. Thus, to eliminate testing constraints, we have optimized three key process variables: RNA extraction and RT-PCR reactions, different sample types and media to facilitate SARS-CoV-2 testing. By performing various validation and bridging studies, we have shown that various sample types such as nasopharyngeal swab, bronchioalveolar lavage and saliva, collected using conventional nasopharyngeal swabs, ESwab or 3D-printed swabs and, preserved in viral transport media, universal transport media, 0.9% sodium chloride or Amies media are compatible with RT-PCR assay for COVID-19. Besides, the reduction of PCR reagents by up to fourfold also produces reliable results. Twit Text FOAVip Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints ????Future Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33179525 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33179525 #FOAvip English Wikipedia <ref>{{Cite pmid|33179525}}</ref> Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints #MMPMID33179525 S Sahajpal N; Mondal AK; Njau A; Ananth S; Jones K; Ahluwalia PK; Ahluwalia M; Jilani Y; Chaubey A; Hegde M; Kota V; Rojiani A; Kolhe R Future Microbiol 2020[Nov]; ä (ä): 1483-1487 PMID33179525show ga RT-PCR-based assays for the detection of SARS-CoV-2 have played an essential role in the current COVID-19 pandemic. However, the sample collection and test reagents are in short supply, primarily due to supply chain issues. Thus, to eliminate testing constraints, we have optimized three key process variables: RNA extraction and RT-PCR reactions, different sample types and media to facilitate SARS-CoV-2 testing. By performing various validation and bridging studies, we have shown that various sample types such as nasopharyngeal swab, bronchioalveolar lavage and saliva, collected using conventional nasopharyngeal swabs, ESwab or 3D-printed swabs and, preserved in viral transport media, universal transport media, 0.9% sodium chloride or Amies media are compatible with RT-PCR assay for COVID-19. Besides, the reduction of PCR reagents by up to fourfold also produces reliable results. äEffective optimization of SARS-CoV-2 laboratory testing variables in a(epmc).pdf DeepDyve Pubget Overpricing