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10.1186/s13567-020-00865-y http://scihub22266oqcxt.onion/10.1186/s13567-020-00865-y 33176871!7656508!33176871     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Vet+Res 2020 ; 51 (1): 136 Nephropedia Template TP {{tp|p=33176871|t=2020. Tomatidine inhibits porcine epidemic diarrhea virus replication by targeting 3CL protease |pdf=|usr=}}{{33176871}} gab.com Text Tomatidine inhibits porcine epidemic diarrhea virus replication by targeting 3CL protease JO: Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=33176871 #FOAvip Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in suckling piglets, leading to severe economic losses worldwide. There is an urgent need to find new therapeutic methods to prevent and control PEDV. Not only is there a shortage of commercial anti-PEDV drugs, but available commercial vaccines fail to protect against highly virulent PEDV variants. We screened an FDA-approved library of 911 natural products and found that tomatidine, a steroidal alkaloid extracted from the skin and leaves of tomatoes, demonstrates significant inhibition of PEDV replication in Vero and IPEC-J2 cells in vitro. Molecular docking and molecular dynamics analysis predicted interactions between tomatidine and the active pocket of PEDV 3CL protease, which were confirmed by fluorescence spectroscopy and isothermal titration calorimetry (ITC). The inhibiting effect of tomatidine on 3CL protease was determined using cleavage visualization and FRET assay. Tomatidine-mediated blocking of 3CL protease activity in PEDV-infected cells was examined by western blot detection of the viral polyprotein in PEDV-infected cells. It indicates that tomatidine inhibits PEDV replication mainly by targeting 3CL protease. In addition, tomatidine also has antiviral activity against transmissible gastroenteritis virus (TGEV), porcine reproductive and respiratory syndrome virus (PRRSV), encephalo myocarditis virus (EMCV) and seneca virus A (SVA) in vitro. These results may be helpful in developing a new prophylactic and therapeutic strategy against PEDV and other swine disease infections. Twit Text FOAVip Tomatidine inhibits porcine epidemic diarrhea virus replication by targeting 3CL protease ????Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=33176871 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33176871 #FOAvip English Wikipedia <ref>{{Cite pmid|33176871}}</ref> Tomatidine inhibits porcine epidemic diarrhea virus replication by targeting 3CL protease #MMPMID33176871 Wang P; Bai J; Liu X; Wang M; Wang X; Jiang P Vet Res 2020[Nov]; 51 (1): 136 PMID33176871show ga Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in suckling piglets, leading to severe economic losses worldwide. There is an urgent need to find new therapeutic methods to prevent and control PEDV. Not only is there a shortage of commercial anti-PEDV drugs, but available commercial vaccines fail to protect against highly virulent PEDV variants. We screened an FDA-approved library of 911 natural products and found that tomatidine, a steroidal alkaloid extracted from the skin and leaves of tomatoes, demonstrates significant inhibition of PEDV replication in Vero and IPEC-J2 cells in vitro. Molecular docking and molecular dynamics analysis predicted interactions between tomatidine and the active pocket of PEDV 3CL protease, which were confirmed by fluorescence spectroscopy and isothermal titration calorimetry (ITC). The inhibiting effect of tomatidine on 3CL protease was determined using cleavage visualization and FRET assay. Tomatidine-mediated blocking of 3CL protease activity in PEDV-infected cells was examined by western blot detection of the viral polyprotein in PEDV-infected cells. It indicates that tomatidine inhibits PEDV replication mainly by targeting 3CL protease. In addition, tomatidine also has antiviral activity against transmissible gastroenteritis virus (TGEV), porcine reproductive and respiratory syndrome virus (PRRSV), encephalo myocarditis virus (EMCV) and seneca virus A (SVA) in vitro. These results may be helpful in developing a new prophylactic and therapeutic strategy against PEDV and other swine disease infections. |Antiviral Agents/chemistry/*pharmacology[MESH] |Peptide Hydrolases/metabolism[MESH] |Plant Extracts/chemistry/pharmacology[MESH] |Porcine epidemic diarrhea virus/drug effects/enzymology/*physiology[MESH] |Tomatine/*analogs & derivatives/chemistry/pharmacology[MESH] |Viral Proteins/*metabolism[MESH]Tomatidine inhibits porcine epidemic diarrhea virus replication by tar(epmc).pdf DeepDyve Pubget Overpricing