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10.1016/j.jcv.2020.104684

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suck abstract from ncbi


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pmid33176236      J+Clin+Virol 2020 ; 133 (ä): 104684
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  • Analytical sensitivity and clinical sensitivity of the three rapid antigen detection kits for detection of SARS-CoV-2 virus #MMPMID33176236
  • Mak GC; Lau SS; Wong KK; Chow NL; Lau CS; Lam ET; Chan RC; Tsang DN
  • J Clin Virol 2020[Dec]; 133 (ä): 104684 PMID33176236show ga
  • BACKGROUND: Numerous rapid antigen detection (RAD) kits for diagnosing COVID-19 patients are available in the market recently. OBJECTIVE: To compare analytical sensitivity and clinical sensitivity for the three commercially available RAD kits. STUDY DESIGN: Analytical sensitivity for the detection of SARS-CoV-2 virus was determined by limit of detection (LOD) using RT-PCR as a reference method. Clinical sensitivity was evaluated by using respiratory specimens collected from confirmed COVID-19 patients. RESULTS: The LOD results showed that the three RAD kits varied from 10(2)-10(5) fold less sensitive than RT-PCR. Clinical sensitivity of RAD kits ranged from 22.9 %-71.4 % for detecting specimens from COVID-19 patients. CONCLUSIONS: Although RAD kits were less sensitive than RT-PCR, understanding the clinical characteristics of different RAD kits can guide us to obtain suitable specimens for testing. The likelihood of positive results for RAD kits will be higher.
  • |Antigens, Viral/*analysis[MESH]
  • |COVID-19 Nucleic Acid Testing[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Limit of Detection[MESH]
  • |Nasopharynx/*virology[MESH]
  • |Reagent Kits, Diagnostic[MESH]
  • |SARS-CoV-2/isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]


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