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10.3390/ijms21218329 http://scihub22266oqcxt.onion/10.3390/ijms21218329 33172005!7664229!33172005     free     free     free       Int+J+Mol+Sci 2020 ; 21 (21): ä Nephropedia Template TP {{tp|p=33172005|t=2020. Tn5 Transposase Applied in Genomics Research |pdf=|usr=}}{{33172005}} gab.com Text Tn5 Transposase Applied in Genomics Research JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33172005 #FOAvip The development of high-throughput sequencing (next-generation sequencing technology (NGS)) and the continuous increase in experimental throughput require the upstream sample processing steps of NGS to be as simple as possible to improve the efficiency of the entire NGS process. The transposition system has fast "cut and paste" and "copy and paste" functions, and has been innovatively applied to the NGS field. For example, the Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) uses high-throughput sequencing to detect chromatin regions accessible by Tn5 transposase. Linear Amplification via Transposon Insertion (LIANTI) uses Tn5 transposase for linear amplification, haploid typing, and structural variation detection. Not only is it efficient and simple, it effectively shortens the time for NGS sample library construction, realizes large-scale and rapid sequencing, improves sequencing resolution, and can be flexibly modified for more technological innovation. Twit Text FOAVip Tn5 Transposase Applied in Genomics Research ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33172005 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33172005 #FOAvip English Wikipedia <ref>{{Cite pmid|33172005}}</ref> Tn5 Transposase Applied in Genomics Research #MMPMID33172005 Li N; Jin K; Bai Y; Fu H; Liu L; Liu B Int J Mol Sci 2020[Nov]; 21 (21): ä PMID33172005show ga The development of high-throughput sequencing (next-generation sequencing technology (NGS)) and the continuous increase in experimental throughput require the upstream sample processing steps of NGS to be as simple as possible to improve the efficiency of the entire NGS process. The transposition system has fast "cut and paste" and "copy and paste" functions, and has been innovatively applied to the NGS field. For example, the Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) uses high-throughput sequencing to detect chromatin regions accessible by Tn5 transposase. Linear Amplification via Transposon Insertion (LIANTI) uses Tn5 transposase for linear amplification, haploid typing, and structural variation detection. Not only is it efficient and simple, it effectively shortens the time for NGS sample library construction, realizes large-scale and rapid sequencing, improves sequencing resolution, and can be flexibly modified for more technological innovation. |Animals[MESH] |Chromatin/genetics[MESH] |Epigenomics/methods[MESH] |Genetic Variation/genetics[MESH] |Genomics/methods[MESH] |High-Throughput Nucleotide Sequencing/*methods[MESH] |Humans[MESH] |Sequence Analysis, DNA/methods[MESH]Tn5 Transposase Applied in Genomics Research (epmc).pdf DeepDyve Pubget Overpricing