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10.1093/nar/gkaa1013 http://scihub22266oqcxt.onion/10.1093/nar/gkaa1013 33167030!7736788!33167030     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2020 ; 48 (22): 12415-12435 Nephropedia Template TP {{tp|p=33167030|t=2020. Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy |pdf=|usr=}}{{33167030}} gab.com Text Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=33167030 #FOAvip The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5' end, the ribosomal frameshift segment and the 3'-untranslated region (3'-UTR) of the SCoV2 genome, their large-scale production and NMR-based secondary structure determination. The NMR data are corroborated with secondary structure probing by DMS footprinting experiments. The close agreement of NMR secondary structure determination of isolated RNA elements with DMS footprinting and NMR performed on larger RNA regions shows that the secondary structure elements fold independently. The NMR data reported here provide the basis for NMR investigations of RNA function, RNA interactions with viral and host proteins and screening campaigns to identify potential RNA binders for pharmaceutical intervention. Twit Text FOAVip Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=33167030 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33167030 #FOAvip English Wikipedia <ref>{{Cite pmid|33167030}}</ref> Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy #MMPMID33167030 Wacker A; Weigand JE; Akabayov SR; Altincekic N; Bains JK; Banijamali E; Binas O; Castillo-Martinez J; Cetiner E; Ceylan B; Chiu LY; Davila-Calderon J; Dhamotharan K; Duchardt-Ferner E; Ferner J; Frydman L; Furtig B; Gallego J; Grun JT; Hacker C; Haddad C; Hahnke M; Hengesbach M; Hiller F; Hohmann KF; Hymon D; de Jesus V; Jonker H; Keller H; Knezic B; Landgraf T; Lohr F; Luo L; Mertinkus KR; Muhs C; Novakovic M; Oxenfarth A; Palomino-Schatzlein M; Petzold K; Peter SA; Pyper DJ; Qureshi NS; Riad M; Richter C; Saxena K; Schamber T; Scherf T; Schlagnitweit J; Schlundt A; Schnieders R; Schwalbe H; Simba-Lahuasi A; Sreeramulu S; Stirnal E; Sudakov A; Tants JN; Tolbert BS; Vogele J; Weiss L; Wirmer-Bartoschek J; Wirtz Martin MA; Wohnert J; Zetzsche H Nucleic Acids Res 2020[Dec]; 48 (22): 12415-12435 PMID33167030show ga The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5' end, the ribosomal frameshift segment and the 3'-untranslated region (3'-UTR) of the SCoV2 genome, their large-scale production and NMR-based secondary structure determination. The NMR data are corroborated with secondary structure probing by DMS footprinting experiments. The close agreement of NMR secondary structure determination of isolated RNA elements with DMS footprinting and NMR performed on larger RNA regions shows that the secondary structure elements fold independently. The NMR data reported here provide the basis for NMR investigations of RNA function, RNA interactions with viral and host proteins and screening campaigns to identify potential RNA binders for pharmaceutical intervention. |*Nucleic Acid Conformation[MESH] |3' Untranslated Regions/genetics[MESH] |Base Sequence[MESH] |COVID-19/epidemiology/*prevention & control/virology[MESH] |Frameshifting, Ribosomal/genetics[MESH] |Genome, Viral/genetics[MESH] |Humans[MESH] |Magnetic Resonance Spectroscopy/*methods[MESH] |Models, Molecular[MESH] |Pandemics[MESH] |RNA, Viral/*chemistry[MESH]Secondary structure determination of conserved SARS-CoV-2 RNA elements(epmc).pdf DeepDyve Pubget Overpricing