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10.1093/nar/gkaa1053

http://scihub22266oqcxt.onion/10.1093/nar/gkaa1053
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33166999!7736786!33166999
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suck abstract from ncbi


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pmid33166999      Nucleic+Acids+Res 2020 ; 48 (22): 12436-12452
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  • Genome-wide mapping of SARS-CoV-2 RNA structures identifies therapeutically-relevant elements #MMPMID33166999
  • Manfredonia I; Nithin C; Ponce-Salvatierra A; Ghosh P; Wirecki TK; Marinus T; Ogando NS; Snijder EJ; van Hemert MJ; Bujnicki JM; Incarnato D
  • Nucleic Acids Res 2020[Dec]; 48 (22): 12436-12452 PMID33166999show ga
  • SARS-CoV-2 is a betacoronavirus with a linear single-stranded, positive-sense RNA genome, whose outbreak caused the ongoing COVID-19 pandemic. The ability of coronaviruses to rapidly evolve, adapt, and cross species barriers makes the development of effective and durable therapeutic strategies a challenging and urgent need. As for other RNA viruses, genomic RNA structures are expected to play crucial roles in several steps of the coronavirus replication cycle. Despite this, only a handful of functionally-conserved coronavirus structural RNA elements have been identified to date. Here, we performed RNA structure probing to obtain single-base resolution secondary structure maps of the full SARS-CoV-2 coronavirus genome both in vitro and in living infected cells. Probing data recapitulate the previously described coronavirus RNA elements (5' UTR and s2m), and reveal new structures. Of these, approximately 10.2% show significant covariation among SARS-CoV-2 and other coronaviruses, hinting at their functionally-conserved role. Secondary structure-restrained 3D modeling of these segments further allowed for the identification of putative druggable pockets. In addition, we identify a set of single-stranded segments in vivo, showing high sequence conservation, suitable for the development of antisense oligonucleotide therapeutics. Collectively, our work lays the foundation for the development of innovative RNA-targeted therapeutic strategies to fight SARS-related infections.
  • |*Nucleic Acid Conformation[MESH]
  • |5' Untranslated Regions/genetics[MESH]
  • |Algorithms[MESH]
  • |Antiviral Agents/chemistry/metabolism/therapeutic use[MESH]
  • |Base Sequence[MESH]
  • |Binding Sites/genetics[MESH]
  • |COVID-19/epidemiology/*prevention & control/virology[MESH]
  • |Conserved Sequence/genetics[MESH]
  • |Genome, Viral/*genetics[MESH]
  • |Humans[MESH]
  • |Models, Molecular[MESH]
  • |Pandemics[MESH]
  • |RNA, Viral/*chemistry[MESH]


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  • suck abstract from ncbi

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