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10.1016/j.meegid.2020.104625 http://scihub22266oqcxt.onion/10.1016/j.meegid.2020.104625 33166683!7648195!33166683     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33166683&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Infect+Genet+Evol 2020 ; 86 (ä): 104625 Nephropedia Template TP {{tp|p=33166683|t=2020. Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2 |pdf=|usr=}}{{33166683}} gab.com Text Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2 JO: Infect Genet Evol http://www.kidney.de/pget.php?&tw=1&pmid=33166683 #FOAvip In late 2019, an outbreak of respiratory disease named COVID-19 started in the world. To date, thousands of cases of infection are reported worldwide. Most researchers focused on epidemiology and clinical features of COVID-19, and a small part of studies was performed to evaluate the genetic characteristics of this virus. Regarding the high price and low availability of sequencing techniques in developing countries, here we describe a rapid and inexpensive method for the detection of D614G mutation in SARS-CoV-2. Using bioinformatics databases and software, we designed the PCR-RFLP method for D614G mutation detection. We evaluated 144 SARS-CoV-2 positive samples isolated in six months in Northeastern Iran. Our results showed that the prevalent type is S-D in our isolates, and a small number of isolated belongs to the S-G type. Of 144 samples, 127 (88.2%) samples have belonged to type S-D, and 13 (9%) samples typed S-G. The first S-G type was detected on 2020 June 10. We have little information about the prevalence of D614G mutation, and it seems that the reason is the lack of cheap and fast methods. We hope that this method will provide more information on the prevalence and epidemiology of D614G mutations worldwide. Twit Text FOAVip Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2 ????Infect Genet Evol http://www.kidney.de/pget.php?&tw=1&pmid=33166683 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33166683 #FOAvip English Wikipedia <ref>{{Cite pmid|33166683}}</ref> Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2 #MMPMID33166683 Hashemi SA; Khoshi A; Ghasemzadeh-Moghaddam H; Ghafouri M; Taghavi M; Namdar-Ahmadabad H; Azimian A Infect Genet Evol 2020[Dec]; 86 (ä): 104625 PMID33166683show ga In late 2019, an outbreak of respiratory disease named COVID-19 started in the world. To date, thousands of cases of infection are reported worldwide. Most researchers focused on epidemiology and clinical features of COVID-19, and a small part of studies was performed to evaluate the genetic characteristics of this virus. Regarding the high price and low availability of sequencing techniques in developing countries, here we describe a rapid and inexpensive method for the detection of D614G mutation in SARS-CoV-2. Using bioinformatics databases and software, we designed the PCR-RFLP method for D614G mutation detection. We evaluated 144 SARS-CoV-2 positive samples isolated in six months in Northeastern Iran. Our results showed that the prevalent type is S-D in our isolates, and a small number of isolated belongs to the S-G type. Of 144 samples, 127 (88.2%) samples have belonged to type S-D, and 13 (9%) samples typed S-G. The first S-G type was detected on 2020 June 10. We have little information about the prevalence of D614G mutation, and it seems that the reason is the lack of cheap and fast methods. We hope that this method will provide more information on the prevalence and epidemiology of D614G mutations worldwide. |COVID-19/virology[MESH] |Humans[MESH] |Mutation/*genetics[MESH] |Polymerase Chain Reaction/*methods[MESH] |Polymorphism, Restriction Fragment Length/*genetics[MESH] |SARS-CoV-2/*genetics[MESH]Development of a PCR-RFLP method for detection of D614G mutation in SA(epmc).pdf DeepDyve Pubget Overpricing