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10.1016/j.vaccine.2020.10.039 http://scihub22266oqcxt.onion/10.1016/j.vaccine.2020.10.039 33164796!7571903!33164796     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Vaccine 2020 ; 38 (49): 7697-7701 Nephropedia Template TP {{tp|p=33164796|t=2020. Identification of a dominant CD8(+) CTL epitope in the SARS-associated coronavirus 2 spike protein |pdf=|usr=}}{{33164796}} gab.com Text Identification of a dominant CD8(+) CTL epitope in the SARS-associated coronavirus 2 spike protein JO: Vaccine http://www.kidney.de/pget.php?&tw=1&pmid=33164796 #FOAvip Coronavirus disease 2019 (COVID-19), which is caused by SARS-CoV-2, has been spreading throughout the world. To date, there are still no approved human vaccines for this disease. To develop an effective vaccine, the establishment of animal models for evaluating post-vaccination immune responses is necessary. In this study, we have identified a CTL epitope in the SARS-CoV-2 spike (S) protein that could be used to measure the cellular immune response against this protein. Potential predicted CTL epitopes of the SARS-CoV-2 S protein were investigated by immunizing BALB/c mice with a recombinant of the receptor-binding domain (RBD) of the S protein. Then, CD8(+) T cells specific for S-RBD were detected by stimulating with potential epitope peptides and then measuring the interferon-gamma production. Truncation of this peptide revealed that S-RBD-specific CD8(+) T cells recognized a H2-D(d)-restricted S(526-533) peptide. In conclusion, this animal model is suitable for evaluating the immunogenicity of SARS-CoV-2 vaccines. Twit Text FOAVip Identification of a dominant CD8(+) CTL epitope in the SARS-associated coronavirus 2 spike protein ????Vaccine http://www.kidney.de/pget.php?&tw=1&pmid=33164796 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33164796 #FOAvip English Wikipedia <ref>{{Cite pmid|33164796}}</ref> Identification of a dominant CD8(+) CTL epitope in the SARS-associated coronavirus 2 spike protein #MMPMID33164796 Muraoka D; Situo D; Sawada SI; Akiyoshi K; Harada N; Ikeda H Vaccine 2020[Nov]; 38 (49): 7697-7701 PMID33164796show ga Coronavirus disease 2019 (COVID-19), which is caused by SARS-CoV-2, has been spreading throughout the world. To date, there are still no approved human vaccines for this disease. To develop an effective vaccine, the establishment of animal models for evaluating post-vaccination immune responses is necessary. In this study, we have identified a CTL epitope in the SARS-CoV-2 spike (S) protein that could be used to measure the cellular immune response against this protein. Potential predicted CTL epitopes of the SARS-CoV-2 S protein were investigated by immunizing BALB/c mice with a recombinant of the receptor-binding domain (RBD) of the S protein. Then, CD8(+) T cells specific for S-RBD were detected by stimulating with potential epitope peptides and then measuring the interferon-gamma production. Truncation of this peptide revealed that S-RBD-specific CD8(+) T cells recognized a H2-D(d)-restricted S(526-533) peptide. In conclusion, this animal model is suitable for evaluating the immunogenicity of SARS-CoV-2 vaccines. |Animals[MESH] |CD8-Positive T-Lymphocytes/*immunology[MESH] |Epitopes, T-Lymphocyte/*immunology/metabolism[MESH] |Female[MESH] |Mice, Inbred BALB C[MESH] |Peptides/immunology/pharmacology[MESH]Identification of a dominant CD8(+) CTL epitope in the SARS-associated(epmc).pdf DeepDyve Pubget Overpricing