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10.1016/j.jcv.2020.104681

http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104681
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suck abstract from ncbi


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pmid33160178      J+Clin+Virol 2020 ; 133 (ä): 104681
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  • First performance report of QIAreach Anti-SARS-CoV-2 Total Test, an innovative nanoparticle fluorescence digital detection platform #MMPMID33160178
  • Stieber F; Howard J; Rao SN; Kawamura LM; Manissero D; Love J; Yang M; Uchiyama R; Parsons S; Miller C; Douwes H; McDonald A; Fairburn L; Boyle J
  • J Clin Virol 2020[Dec]; 133 (ä): 104681 PMID33160178show ga
  • In 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a global pandemic. Disease diagnosis, appropriate clinical management and infection control are all important factors in controlling the spread of SARS-CoV-2. The QIAreach Anti-SARS-CoV-2 Total Test (Anti-CoV2) is a rapid, qualitative serological test, using proprietary nanoparticle fluorescence technology to detect total antibody (IgA, IgM, and IgG) against SARS-CoV-2. Here we report the results of the US Food and Drug Administration (FDA) clinical agreement study. Thirty positive plasma or serum samples were taken from consenting individuals with polymerase chain reaction (PCR)-confirmed SARS-CoV-2 infection >/=14 days from symptom onset. Seventy-five samples from before the believed circulation of SARS-CoV-2 (November 1, 2019) were used to assess specificity. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated along with the corresponding exact two-sided 95 % confidence intervals (CI) using an FDA Emergency Use Authorized PCR test as the reference method. Anti-CoV2 was shown to have 100 % sensitivity (PPA; 95 % CI 88.4-100 %) and 100 % specificity (NPA; 95 % CI 95.2-100 %). Against 157 pre-pandemic samples, no cross-reactivity was observed with seasonal coronaviruses or other respiratory pathogens tested. Additionally, no interference was observed when samples were spiked with: conjugated bilirubin 0.4 mg/ml; unconjugated bilirubin 0.4 mg/ml; hemoglobin 5 mg/ml; prednisolone 0.12 mg/ml; triglycerides 15 mg/ml. In conclusion, Anti-CoV2 provides accurate qualitative detection of total antibodies against SARS-CoV-2.
  • |*Fluorescence[MESH]
  • |*Nanoparticles[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Digital Technology[MESH]
  • |Humans[MESH]
  • |Immunoglobulin A/blood[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Immunoglobulin M/blood[MESH]
  • |Sensitivity and Specificity[MESH]
  • |United States[MESH]


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