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10.1016/j.jviromet.2020.114012 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.114012 33157149!ä!33157149 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 288 (ä): 114012 Nephropedia Template TP {{tp|p=33157149|t=2021. Development of SYBR Green I-based real-time reverse transcription polymerase chain reaction for the detection of feline astrovirus |pdf=|usr=}}{{33157149}} gab.com Text Development of SYBR Green I-based real-time reverse transcription polymerase chain reaction for the detection of feline astrovirus JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33157149 #FOAvip In this study, a SYBR Green I-based real-time reverse transcription-polymerase chain reaction (RT-PCR) was developed for the clinical diagnosis of feline astroviruses (FeAstVs). Specific primers were designed based on the conserved region of the FeAstV ORF1b gene. Experiments for specificity, sensitivity, and repeatability of the assay were carried out. In addition, the assay was evaluated using clinical samples. Specificity analysis indicated that the assay showed negative results with samples of Feline Parvovirus, Feline Herpesvirus, Feline Calicivirus, Feline Bocavirus, and Feline Coronavirus, indicating good specificity of the assay. Sensitivity analysis showed that the SYBR Green I-based real-time RT-PCR method could detect as low as 3.72 x 10(1) copies/muL of template, which is 100-fold more sensitive compared to the conventional RT-PCR. Both intra-assay and inter-assay variability were lower than 1 %, indicating good reproducibility. Furthermore, an analysis of 150 fecal samples showed that the positive detection rate of SYBR Green I-based real-time RT-PCR was higher than that of the conventional RT-PCR, indicating the high reliability of the method. The assay is cheap and effective. Therefore, it could provide support for the detection of FeAstV in large-scale clinical testing and epidemiological investigation. Twit Text FOAVip Development of SYBR Green I-based real-time reverse transcription polymerase chain reaction for the detection of feline astrovirus ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33157149 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33157149 #FOAvip English Wikipedia <ref>{{Cite pmid|33157149}}</ref> Development of SYBR Green I-based real-time reverse transcription polymerase chain reaction for the detection of feline astrovirus #MMPMID33157149 Wang Y; Fu Z; Guo X; Zhang D; Bai C; Li W; Liu G; Li Y; Jiang S J Virol Methods 2021[Feb]; 288 (ä): 114012 PMID33157149show ga In this study, a SYBR Green I-based real-time reverse transcription-polymerase chain reaction (RT-PCR) was developed for the clinical diagnosis of feline astroviruses (FeAstVs). Specific primers were designed based on the conserved region of the FeAstV ORF1b gene. Experiments for specificity, sensitivity, and repeatability of the assay were carried out. In addition, the assay was evaluated using clinical samples. Specificity analysis indicated that the assay showed negative results with samples of Feline Parvovirus, Feline Herpesvirus, Feline Calicivirus, Feline Bocavirus, and Feline Coronavirus, indicating good specificity of the assay. Sensitivity analysis showed that the SYBR Green I-based real-time RT-PCR method could detect as low as 3.72 x 10(1) copies/muL of template, which is 100-fold more sensitive compared to the conventional RT-PCR. Both intra-assay and inter-assay variability were lower than 1 %, indicating good reproducibility. Furthermore, an analysis of 150 fecal samples showed that the positive detection rate of SYBR Green I-based real-time RT-PCR was higher than that of the conventional RT-PCR, indicating the high reliability of the method. The assay is cheap and effective. Therefore, it could provide support for the detection of FeAstV in large-scale clinical testing and epidemiological investigation. |*Organic Chemicals[MESH] |*Real-Time Polymerase Chain Reaction/methods/standards[MESH] |Animals[MESH] |Astroviridae/*genetics[MESH] |Benzothiazoles[MESH] |Cat Diseases/*diagnosis/*virology[MESH] |Cats[MESH] |Diamines[MESH] |Quinolines[MESH] |Reproducibility of Results[MESH]Development of SYBR Green I-based real-time reverse transcription poly(epmc).pdf DeepDyve Pubget Overpricing