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10.1177/1535370220963793 http://scihub22266oqcxt.onion/10.1177/1535370220963793 33153299!7885046!33153299     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Exp+Biol+Med+(Maywood) 2021 ; 246 (4): 400-405 Nephropedia Template TP {{tp|p=33153299|t=2021. Detection of severe acute respiratory syndrome coronavirus 2 and influenza viruses based on CRISPR-Cas12a |pdf=|usr=}}{{33153299}} gab.com Text Detection of severe acute respiratory syndrome coronavirus 2 and influenza viruses based on CRISPR-Cas12a JO: Exp Biol Med (Maywood) http://www.kidney.de/pget.php?&tw=1&pmid=33153299 #FOAvip Due to the common symptoms of COVID-19, patients are similar to influenza-like illness. Therefore, the detection method would be crucial to discriminate between SARS-CoV-2 and influenza virus-infected patients. In this study, CRISPR-Cas12a-based detection was applied for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus, and influenza B virus which would be a practical and attractive application for screening of patients with COVID-19 and influenza in areas with limited resources. The limit of detection for SARS-CoV-2, influenza A, and influenza B detection was 10, 10(3), and 10(3) copies/reaction, respectively. Moreover, the assays yielded no cross-reactivity against other respiratory viruses. The results revealed that the detection of influenza virus and SARS-CoV-2 by using RT-RPA and CRISPR-Cas12a technology reaches 96.23% sensitivity and 100% specificity for SARS-CoV-2 detection. The sensitivity for influenza virus A and B detections was 85.07% and 94.87%, respectively. In addition, the specificity for influenza virus A and B detections was approximately 96%. In conclusion, the RT-RPA with CRISPR-Cas12a assay was an effective method for the screening of influenza viruses and SARS-CoV-2 which could be applied to detect other infectious diseases in the future. Twit Text FOAVip Detection of severe acute respiratory syndrome coronavirus 2 and influenza viruses based on CRISPR-Cas12a ????Exp Biol Med (Maywood) http://www.kidney.de/pget.php?&tw=1&pmid=33153299 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33153299 #FOAvip English Wikipedia <ref>{{Cite pmid|33153299}}</ref> Detection of severe acute respiratory syndrome coronavirus 2 and influenza viruses based on CRISPR-Cas12a #MMPMID33153299 Mayuramart O; Nimsamer P; Rattanaburi S; Chantaravisoot N; Khongnomnan K; Chansaenroj J; Puenpa J; Suntronwong N; Vichaiwattana P; Poovorawan Y; Payungporn S Exp Biol Med (Maywood) 2021[Feb]; 246 (4): 400-405 PMID33153299show ga Due to the common symptoms of COVID-19, patients are similar to influenza-like illness. Therefore, the detection method would be crucial to discriminate between SARS-CoV-2 and influenza virus-infected patients. In this study, CRISPR-Cas12a-based detection was applied for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus, and influenza B virus which would be a practical and attractive application for screening of patients with COVID-19 and influenza in areas with limited resources. The limit of detection for SARS-CoV-2, influenza A, and influenza B detection was 10, 10(3), and 10(3) copies/reaction, respectively. Moreover, the assays yielded no cross-reactivity against other respiratory viruses. The results revealed that the detection of influenza virus and SARS-CoV-2 by using RT-RPA and CRISPR-Cas12a technology reaches 96.23% sensitivity and 100% specificity for SARS-CoV-2 detection. The sensitivity for influenza virus A and B detections was 85.07% and 94.87%, respectively. In addition, the specificity for influenza virus A and B detections was approximately 96%. In conclusion, the RT-RPA with CRISPR-Cas12a assay was an effective method for the screening of influenza viruses and SARS-CoV-2 which could be applied to detect other infectious diseases in the future. |*Molecular Diagnostic Techniques[MESH] |*Nucleic Acid Amplification Techniques[MESH] |COVID-19/*diagnosis[MESH] |CRISPR-Cas Systems[MESH] |Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |Humans[MESH] |Influenza A virus/*genetics/isolation & purification[MESH] |Influenza B virus/*genetics/isolation & purification[MESH] |Influenza, Human/*diagnosis[MESH] |Limit of Detection[MESH] |RNA, Viral/analysis/genetics[MESH]Detection of severe acute respiratory syndrome coronavirus 2 and influ(epmc).pdf DeepDyve Pubget Overpricing