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10.1016/j.jviromet.2020.114009

http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.114009
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33152411!7605757!33152411
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suck abstract from ncbi


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pmid33152411      J+Virol+Methods 2021 ; 287 (ä): 114009
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  • A high-throughput Anti-SARS-CoV-2 IgG testing platform for COVID-19 #MMPMID33152411
  • Du J; Chu E; Zhang D; Lu CM; Zhang A; Sha MY
  • J Virol Methods 2021[Jan]; 287 (ä): 114009 PMID33152411show ga
  • BACKGROUND: Serology tests for detecting the antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can identify previous infection and help to confirm the presence of current infection. OBJECTIVE: The aim of this study was to evaluate the performances of a newly developed high throughput immunoassay for anti-SARS-CoV-2 IgG antibody detection. RESULTS: Clinical agreement studies were performed in 107 COVID-19 patient serum samples and 226 negative donor serum/plasma samples. Positive percent agreement (PPA) was 46.15 % (95 % CI: 19.22 % approximately 74.87 %), 61.54 % (95 % CI: 31.58 % approximately 86.14 %), and 97.53 % (95 % CI: 91.36 % approximately 99.70 %) for samples collected on 0-7 days, 8-14 days, and >/=15 days from symptom onset, respectively. Negative Percent Agreement (NPA) was 98.23 % (95 % CI: 95.53 % approximately 99.52 %). No cross-reactivity was observed to patient samples positive for IgG antibodies against the following pathogens: HIV, HAV, HBV, RSV, CMV, EBV, Rubella, Influenza A, and Influenza B. Hemoglobin (200 mg/dL), bilirubin (2 mg/dL) and EDTA (10 mM) showed no significant interfering effect on this assay. CONCLUSION: An anti-SARS-CoV-2 IgG antibody assay with high sensitivity and specificity has been developed. With the high throughput, this assay will speed up anti-SARS-CoV-2 IgG testing.
  • |Antibodies, Viral/blood[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/blood/*diagnosis[MESH]
  • |High-Throughput Screening Assays[MESH]
  • |Humans[MESH]
  • |Immunoassay[MESH]
  • |Immunoglobulin G/*blood[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/*immunology/isolation & purification[MESH]


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