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10.4049/jimmunol.2000842 http://scihub22266oqcxt.onion/10.4049/jimmunol.2000842 33148714!ä!33148714 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33148714&cmd=llinks): Failed to open stream: HTTP request failed! 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SARS-CoV-2 Cysteine-like Protease Antibodies Can Be Detected in Serum and Saliva of COVID-19-Seropositive Individuals |pdf=|usr=}}{{33148714}} gab.com Text SARS-CoV-2 Cysteine-like Protease Antibodies Can Be Detected in Serum and Saliva of COVID-19-Seropositive Individuals JO: J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=33148714 #FOAvip Currently, there is a need for reliable tests that allow identification of individuals that have been infected with SARS-CoV-2 even if the infection was asymptomatic. To date, the vast majority of the serological tests for SARS-CoV-2-specific Abs are based on serum detection of Abs to either the viral spike glycoprotein (the major target for neutralizing Abs) or the viral nucleocapsid protein that is known to be highly immunogenic in other coronaviruses. Conceivably, exposure of Ags released from infected cells could stimulate Ab responses that might correlate with tissue damage and, hence, they may have some value as a prognostic indicator. We addressed whether other nonstructural viral proteins, not incorporated into the infectious viral particle, specifically the viral cysteine-like protease, might also be potent immunogens. Using ELISA tests, coating several SARS-CoV-2 proteins produced in vitro, we describe that COVID-19 patients make high titer IgG, IgM, and IgA Ab responses to the Cys-like protease from SARS-CoV-2, also known as 3CLpro or Mpro, and it can be used to identify individuals with positive serology against the coronavirus. Higher Ab titers in these assays associated with more-severe disease, and no cross-reactive Abs against prior betacoronavirus were found. Remarkably, IgG Abs specific for Mpro and other SARS-CoV-2 Ags can also be detected in saliva. In conclusion, Mpro is a potent Ag in infected patients that can be used in serological tests, and its detection in saliva could be the basis for a rapid, noninvasive test for COVID-19 seropositivity. Twit Text FOAVip SARS-CoV-2 Cysteine-like Protease Antibodies Can Be Detected in Serum and Saliva of COVID-19-Seropositive Individuals ????J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=33148714 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33148714 #FOAvip English Wikipedia <ref>{{Cite pmid|33148714}}</ref> SARS-CoV-2 Cysteine-like Protease Antibodies Can Be Detected in Serum and Saliva of COVID-19-Seropositive Individuals #MMPMID33148714 Martinez-Fleta P; Alfranca A; Gonzalez-Alvaro I; Casasnovas JM; Fernandez-Soto D; Esteso G; Caceres-Martell Y; Gardeta S; Lopez-Sanz C; Prat S; Mateu-Albero T; Gabrie L; Lopez-Granados E; Sanchez-Madrid F; Reyburn HT; Rodriguez Frade JM; Vales-Gomez M J Immunol 2020[Dec]; 205 (11): 3130-3140 PMID33148714show ga Currently, there is a need for reliable tests that allow identification of individuals that have been infected with SARS-CoV-2 even if the infection was asymptomatic. To date, the vast majority of the serological tests for SARS-CoV-2-specific Abs are based on serum detection of Abs to either the viral spike glycoprotein (the major target for neutralizing Abs) or the viral nucleocapsid protein that is known to be highly immunogenic in other coronaviruses. Conceivably, exposure of Ags released from infected cells could stimulate Ab responses that might correlate with tissue damage and, hence, they may have some value as a prognostic indicator. We addressed whether other nonstructural viral proteins, not incorporated into the infectious viral particle, specifically the viral cysteine-like protease, might also be potent immunogens. Using ELISA tests, coating several SARS-CoV-2 proteins produced in vitro, we describe that COVID-19 patients make high titer IgG, IgM, and IgA Ab responses to the Cys-like protease from SARS-CoV-2, also known as 3CLpro or Mpro, and it can be used to identify individuals with positive serology against the coronavirus. Higher Ab titers in these assays associated with more-severe disease, and no cross-reactive Abs against prior betacoronavirus were found. Remarkably, IgG Abs specific for Mpro and other SARS-CoV-2 Ags can also be detected in saliva. In conclusion, Mpro is a potent Ag in infected patients that can be used in serological tests, and its detection in saliva could be the basis for a rapid, noninvasive test for COVID-19 seropositivity. |Adult[MESH] |Aged[MESH] |Antibodies, Viral/*blood[MESH] |Betacoronavirus/*metabolism[MESH] |COVID-19[MESH] |Coronavirus Infections/*blood[MESH] |Cysteine Proteases/*metabolism[MESH] |Female[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Nucleocapsid Proteins/*metabolism[MESH] |Pandemics[MESH] |Pneumonia, Viral/*blood[MESH] |SARS-CoV-2[MESH]SARS-CoV-2 Cysteine-like Protease Antibodies Can Be Detected in Serum (epmc).pdf DeepDyve Pubget Overpricing