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10.1016/j.stem.2020.10.004 http://scihub22266oqcxt.onion/10.1016/j.stem.2020.10.004 33142113!7577700!33142113     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Stem+Cell 2020 ; 27 (6): 905-919.e10 Nephropedia Template TP {{tp|p=33142113|t=2020. Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2 |pdf=|usr=}}{{33142113}} gab.com Text Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2 JO: Cell Stem Cell http://www.kidney.de/pget.php?&tw=1&pmid=33142113 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the cause of a present pandemic, infects human lung alveolar type 2 (hAT2) cells. Characterizing pathogenesis is crucial for developing vaccines and therapeutics. However, the lack of models mirroring the cellular physiology and pathology of hAT2 cells limits the study. Here, we develop a feeder-free, long-term, three-dimensional (3D) culture technique for hAT2 cells derived from primary human lung tissue and investigate infection response to SARS-CoV-2. By imaging-based analysis and single-cell transcriptome profiling, we reveal rapid viral replication and the increased expression of interferon-associated genes and proinflammatory genes in infected hAT2 cells, indicating a robust endogenous innate immune response. Further tracing of viral mutations acquired during transmission identifies full infection of individual cells effectively from a single viral entry. Our study provides deep insights into the pathogenesis of SARS-CoV-2 and the application of defined 3D hAT2 cultures as models for respiratory diseases. Twit Text FOAVip Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2 ????Cell Stem Cell http://www.kidney.de/pget.php?&tw=1&pmid=33142113 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33142113 #FOAvip English Wikipedia <ref>{{Cite pmid|33142113}}</ref> Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2 #MMPMID33142113 Youk J; Kim T; Evans KV; Jeong YI; Hur Y; Hong SP; Kim JH; Yi K; Kim SY; Na KJ; Bleazard T; Kim HM; Fellows M; Mahbubani KT; Saeb-Parsy K; Kim SY; Kim YT; Koh GY; Choi BS; Ju YS; Lee JH Cell Stem Cell 2020[Dec]; 27 (6): 905-919.e10 PMID33142113show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the cause of a present pandemic, infects human lung alveolar type 2 (hAT2) cells. Characterizing pathogenesis is crucial for developing vaccines and therapeutics. However, the lack of models mirroring the cellular physiology and pathology of hAT2 cells limits the study. Here, we develop a feeder-free, long-term, three-dimensional (3D) culture technique for hAT2 cells derived from primary human lung tissue and investigate infection response to SARS-CoV-2. By imaging-based analysis and single-cell transcriptome profiling, we reveal rapid viral replication and the increased expression of interferon-associated genes and proinflammatory genes in infected hAT2 cells, indicating a robust endogenous innate immune response. Further tracing of viral mutations acquired during transmission identifies full infection of individual cells effectively from a single viral entry. Our study provides deep insights into the pathogenesis of SARS-CoV-2 and the application of defined 3D hAT2 cultures as models for respiratory diseases. |*COVID-19/virology[MESH] |Cell Culture Techniques[MESH] |Culture Media[MESH] |Humans[MESH] |Interferons/metabolism[MESH] |Models, Biological[MESH] |Pulmonary Alveoli/metabolism/ultrastructure/*virology[MESH] |SARS-CoV-2/*physiology/ultrastructure[MESH] |Stem Cells/*virology[MESH] |Transcriptome[MESH] |Virus Internalization[MESH]Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infec(epmc).pdf DeepDyve Pubget Overpricing