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10.1128/JCM.02504-20

http://scihub22266oqcxt.onion/10.1128/JCM.02504-20
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33139421!8111130!33139421
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suck abstract from ncbi


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pmid33139421      J+Clin+Microbiol 2021 ; 59 (2): ä
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  • Evaluation of a SARS-CoV-2 Surrogate Virus Neutralization Test for Detection of Antibody in Human, Canine, Cat, and Hamster Sera #MMPMID33139421
  • Perera RAPM; Ko R; Tsang OTY; Hui DSC; Kwan MYM; Brackman CJ; To EMW; Yen HL; Leung K; Cheng SMS; Chan KH; Chan KCK; Li KC; Saif L; Barrs VR; Wu JT; Sit THC; Poon LLM; Peiris M
  • J Clin Microbiol 2021[Jan]; 59 (2): ä PMID33139421show ga
  • Surrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT(90)) in human, canine, cat, and hamster sera. With PRNT(90) as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT(90), except for cross-reactivity to SARS-CoV-1 in sVNT.
  • |Animals[MESH]
  • |Antibodies, Neutralizing/blood[MESH]
  • |Antibodies, Viral/blood[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/blood/*diagnosis/pathology[MESH]
  • |Cats[MESH]
  • |Cricetinae[MESH]
  • |Cross Reactions[MESH]
  • |Dogs[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immune Sera/immunology[MESH]
  • |Male[MESH]
  • |Neutralization Tests/*methods/standards[MESH]
  • |SARS-CoV-2/immunology/*isolation & purification[MESH]


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