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Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Clin+Microbiol 2021 ; 59 (2): ä Nephropedia Template TP
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Evaluation of a SARS-CoV-2 Surrogate Virus Neutralization Test for Detection of Antibody in Human, Canine, Cat, and Hamster Sera #MMPMID33139421
Perera RAPM; Ko R; Tsang OTY; Hui DSC; Kwan MYM; Brackman CJ; To EMW; Yen HL; Leung K; Cheng SMS; Chan KH; Chan KCK; Li KC; Saif L; Barrs VR; Wu JT; Sit THC; Poon LLM; Peiris M
J Clin Microbiol 2021[Jan]; 59 (2): ä PMID33139421show ga
Surrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT(90)) in human, canine, cat, and hamster sera. With PRNT(90) as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT(90), except for cross-reactivity to SARS-CoV-1 in sVNT.