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10.18632/aging.104020 http://scihub22266oqcxt.onion/10.18632/aging.104020 33136068!7695381!33136068     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Aging+(Albany+NY) 2020 ; 12 (21): 20997-21003 Nephropedia Template TP {{tp|p=33136068|t=2020. Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Virus in discharged COVID-19 patients |pdf=|usr=}}{{33136068}} gab.com Text Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Virus in discharged COVID-19 patients JO: Aging (Albany NY) http://www.kidney.de/pget.php?&tw=1&pmid=33136068 #FOAvip The worldwide severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak has led to the rapid spread of coronavirus disease (COVID-19). The quantitative real time PCR (qPCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, more and more infected patients are relapsing after discharge, which suggests qPCR may fail to detect the virus in some cases. In this study, we selected 74 clinical samples from 43 recovering inpatients for qPCR and Droplet Digital PCR (ddPCR) synchronous blind detection, and established a cutoff value for ddPCR diagnosis of COVID-19. The results showed that at a cutoff value of 0.04 copies/muL, the ddPCR sensitivity and specificity are 97.6% and 100%, respectively. In addition, we also analyzed 18 retained samples from 9 discharged patients who relapsed. Although qPCR showed all 18 samples to be negative, ddPCR showed 12 to be positive, and there was only one patient with two negative samples; the other eight patients had at least one positive sample. These results indicate that ddPCR could significantly improve the accuracy of COVID-19 diagnosis, especially for discharged patients with a low viral load, and help to reduce misdiagnosis during recovery. Twit Text FOAVip Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Virus in discharged COVID-19 patients ????Aging (Albany NY) http://www.kidney.de/pget.php?&tw=1&pmid=33136068 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33136068 #FOAvip English Wikipedia <ref>{{Cite pmid|33136068}}</ref> Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Virus in discharged COVID-19 patients #MMPMID33136068 Liu C; Shi Q; Peng M; Lu R; Li H; Cai Y; Chen J; Xu J; Shen B Aging (Albany NY) 2020[Nov]; 12 (21): 20997-21003 PMID33136068show ga The worldwide severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak has led to the rapid spread of coronavirus disease (COVID-19). The quantitative real time PCR (qPCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, more and more infected patients are relapsing after discharge, which suggests qPCR may fail to detect the virus in some cases. In this study, we selected 74 clinical samples from 43 recovering inpatients for qPCR and Droplet Digital PCR (ddPCR) synchronous blind detection, and established a cutoff value for ddPCR diagnosis of COVID-19. The results showed that at a cutoff value of 0.04 copies/muL, the ddPCR sensitivity and specificity are 97.6% and 100%, respectively. In addition, we also analyzed 18 retained samples from 9 discharged patients who relapsed. Although qPCR showed all 18 samples to be negative, ddPCR showed 12 to be positive, and there was only one patient with two negative samples; the other eight patients had at least one positive sample. These results indicate that ddPCR could significantly improve the accuracy of COVID-19 diagnosis, especially for discharged patients with a low viral load, and help to reduce misdiagnosis during recovery. |*COVID-19 Nucleic Acid Testing/classification/methods/standards[MESH] |*COVID-19/diagnosis/epidemiology/prevention & control[MESH] |China/epidemiology[MESH] |Humans[MESH] |Patient Discharge/*standards[MESH] |Quality Improvement[MESH] |SARS-CoV-2/*isolation & purification[MESH] |Secondary Prevention/*methods[MESH] |Sensitivity and Specificity[MESH]Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Vir(epmc).pdf DeepDyve Pubget Overpricing