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10.3389/fpubh.2020.555013

http://scihub22266oqcxt.onion/10.3389/fpubh.2020.555013
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33134237!7579402!33134237
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suck abstract from ncbi


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pmid33134237      Front+Public+Health 2020 ; 8 (ä): 555013
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  • Identification of a Novel Pathogen Using Family-Wide PCR: Initial Confirmation of COVID-19 in Thailand #MMPMID33134237
  • Wacharapluesadee S; Buathong R; Iamsirithawon S; Chaifoo W; Ponpinit T; Ruchisrisarod C; Sonpee C; Katasrila P; Yomrat S; Ghai S; Sirivichayakul S; Okada P; Mekha N; Karnkawinpong O; Uttayamakul S; Vachiraphan A; Plipat T; Hemachudha T
  • Front Public Health 2020[]; 8 (ä): 555013 PMID33134237show ga
  • In resource-limited countries, early detection of novel pathogens is often challenging, due to financial and technical constraints. This study reports the efficacy of family-wide polymerase chain reaction (PCR) in screening, detecting, and identifying initial cases of the novel SARS-CoV-2 in Thailand. Respiratory secretions were collected from suspected individuals traveling from Wuhan, China to Thailand at the beginning of January 2020. Family-wide PCR assays yielded positive results for coronavirus in one traveler within 12 h on January 8, 2020. Nucleotide sequences (290 bp) showed 100% similarity to SARS-CoV-2. The whole genome sequence was further characterized by Next Generation Sequencing (NGS) for confirmation. Combining family-wide PCR, as a rapid screening tool, with NGS, for full genome characterization, could facilitate early detection and confirmation of a novel pathogen and enable early containment of a disease outbreak.
  • |*COVID-19[MESH]
  • |China[MESH]
  • |Humans[MESH]
  • |Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]


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