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10.1007/s42485-020-00044-9

http://scihub22266oqcxt.onion/10.1007/s42485-020-00044-9
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33132628!7457902!33132628
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suck abstract from ncbi


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pmid33132628      J+Proteins+Proteom 2020 ; 11 (3): 159-165
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  • A rapid and sensitive method to detect SARS-CoV-2 virus using targeted-mass spectrometry #MMPMID33132628
  • Singh P; Chakraborty R; Marwal R; Radhakrishan VS; Bhaskar AK; Vashisht H; Dhar MS; Pradhan S; Ranjan G; Imran M; Raj A; Sharma U; Singh P; Lall H; Dutta M; Garg P; Ray A; Dash D; Sivasubbu S; Gogia H; Madan P; Kabra S; Singh SK; Agrawal A; Rakshit P; Kumar P; Sengupta S
  • J Proteins Proteom 2020[]; 11 (3): 159-165 PMID33132628show ga
  • In the last few months, there has been a global catastrophic outbreak of severe acute respiratory syndrome disease caused by the novel coronavirus SARS-CoV-2 affecting millions of people worldwide. Early diagnosis and isolation are key to contain the rapid spread of the virus. Towards this goal, we report a simple, sensitive and rapid method to detect the virus using a targeted mass spectrometric approach, which can directly detect the presence of virus from naso-oropharyngeal swabs. Using a multiple reaction monitoring we can detect the presence of two peptides specific to SARS-CoV-2 in a 2.3 min gradient run with 100% specificity and 90.5% sensitivity when compared to RT-PCR. Importantly, we further show that these peptides could be detected even in the patients who have recovered from the symptoms and have tested negative for the virus by RT-PCR highlighting the sensitivity of the technique. This method has the translational potential of in terms of the rapid diagnostics of symptomatic and asymptomatic COVID-19 and can augment current methods available for diagnosis of SARS-CoV-2.
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