Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.ijid.2020.10.079 http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.10.079 33130201!7598553!33130201     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Infect+Dis 2021 ; 102 (ä): 437-439 Nephropedia Template TP {{tp|p=33130201|t=2021. Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis |pdf=|usr=}}{{33130201}} gab.com Text Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33130201 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and quickly spread around the world, forcing global health authorities to develop protocols for its diagnosis. Here we report dimer formation in the N2 primers-probe set (CDC 2019-nCoV Real-Time RT-PCR) used in the diagnostic routine, and propose alternatives to reduce dimerization events. Late unspecific amplifications were visualized in 56.4% of negative samples and 57.1% of no-template control, but not in positive samples or positive control. In silico analysis and gel electrophoresis confirmed the dimer formation. The RT-qPCR parameters were optimized and the late unspecific amplifications decreased to 11.5% in negative samples and no-template control. The adjustment of PCR parameters was essential to reduce the risk of false-positives results and to avoid inclusive results requiring repeat testing, which increases the costs and generates delays in results or even unnecessary requests for new samples. Twit Text FOAVip Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33130201 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33130201 #FOAvip English Wikipedia <ref>{{Cite pmid|33130201}}</ref> Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis #MMPMID33130201 Jaeger LH; Nascimento TC; Rocha FD; Vilela FMP; Duque APDN; Silva LM; Riani LR; Moreira JP; Chagas JMA; Pereira TV; Perches CGP; Watanabe ASA; Viccini LF; Silverio MS; Correa JODA; Pereira-Junior ODS; Pittella F Int J Infect Dis 2021[Jan]; 102 (ä): 437-439 PMID33130201show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and quickly spread around the world, forcing global health authorities to develop protocols for its diagnosis. Here we report dimer formation in the N2 primers-probe set (CDC 2019-nCoV Real-Time RT-PCR) used in the diagnostic routine, and propose alternatives to reduce dimerization events. Late unspecific amplifications were visualized in 56.4% of negative samples and 57.1% of no-template control, but not in positive samples or positive control. In silico analysis and gel electrophoresis confirmed the dimer formation. The RT-qPCR parameters were optimized and the late unspecific amplifications decreased to 11.5% in negative samples and no-template control. The adjustment of PCR parameters was essential to reduce the risk of false-positives results and to avoid inclusive results requiring repeat testing, which increases the costs and generates delays in results or even unnecessary requests for new samples. |*SARS-CoV-2[MESH] |COVID-19 Testing[MESH] |COVID-19/*diagnosis[MESH] |DNA Primers[MESH] |Humans[MESH] |RNA, Viral/analysis[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH]Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS(epmc).pdf DeepDyve Pubget Overpricing