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10.1016/j.jviromet.2020.114007 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.114007 33130151!7598561!33130151     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 288 (ä): 114007 Nephropedia Template TP {{tp|p=33130151|t=2021. End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19) |pdf=|usr=}}{{33130151}} gab.com Text End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19) JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33130151 #FOAvip Real-time reverse transcription-polymerase chain reaction (RT-qPCR) is considered the "gold standard" for the direct diagnosis of SARS-CoV-2 infections. However, routine diagnosis by RT-qPCR is a limitation for many laboratories, mainly due to the infrastructure and/or disproportionate relationship between demand and supply of inputs. In this context, and to increase the diagnostic coverage of SARS-CoV-2 infections, we describe an alternative, sensitive and specific one-step end-point RT-PCR for the detection of the SARS-CoV-2 E gene. The performance of the RT-PCR was evaluated in 43 clinical samples, of which 10 and 33 were previously identified as negative and positive, respectively, by RT-qPCR. Among the positive samples, 15 and 18 were from asymptomatic and symptomatic individuals, respectively. Here, 32/33 of the positive samples in the RT-qPCR, including from asymptomatic individuals, were found positive in the RT-PCR (Ct 15.94-34.92). The analytical sensitivity of the assay was about 7.15-9 copies of vRNA/muL, and nonspecific amplifications were not observed in SARS-CoV-2 negative samples. Importantly, the RT-PCR reactions were performed in a 10 muL final volume. Finally, considering specificity, analytical sensitivity and cost reduction, we believe that the RT-PCR platform described here may be a viable option for the diagnostic of SARS-CoV-2 infections in laboratories in which RT-qPCR is not available. Twit Text FOAVip End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19) ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33130151 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33130151 #FOAvip English Wikipedia <ref>{{Cite pmid|33130151}}</ref> End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19) #MMPMID33130151 Silva Junior JVJ; Merchioratto I; de Oliveira PSB; Rocha Lopes TR; Brites PC; de Oliveira EM; Weiblen R; Flores EF J Virol Methods 2021[Feb]; 288 (ä): 114007 PMID33130151show ga Real-time reverse transcription-polymerase chain reaction (RT-qPCR) is considered the "gold standard" for the direct diagnosis of SARS-CoV-2 infections. However, routine diagnosis by RT-qPCR is a limitation for many laboratories, mainly due to the infrastructure and/or disproportionate relationship between demand and supply of inputs. In this context, and to increase the diagnostic coverage of SARS-CoV-2 infections, we describe an alternative, sensitive and specific one-step end-point RT-PCR for the detection of the SARS-CoV-2 E gene. The performance of the RT-PCR was evaluated in 43 clinical samples, of which 10 and 33 were previously identified as negative and positive, respectively, by RT-qPCR. Among the positive samples, 15 and 18 were from asymptomatic and symptomatic individuals, respectively. Here, 32/33 of the positive samples in the RT-qPCR, including from asymptomatic individuals, were found positive in the RT-PCR (Ct 15.94-34.92). The analytical sensitivity of the assay was about 7.15-9 copies of vRNA/muL, and nonspecific amplifications were not observed in SARS-CoV-2 negative samples. Importantly, the RT-PCR reactions were performed in a 10 muL final volume. Finally, considering specificity, analytical sensitivity and cost reduction, we believe that the RT-PCR platform described here may be a viable option for the diagnostic of SARS-CoV-2 infections in laboratories in which RT-qPCR is not available. |*Reverse Transcriptase Polymerase Chain Reaction[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/*virology[MESH] |Humans[MESH] |RNA, Viral[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2/*genetics[MESH]End-point RT-PCR: A potential alternative for diagnosing coronavirus d(epmc).pdf DeepDyve Pubget Overpricing