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10.1093/infdis/jiaa680

http://scihub22266oqcxt.onion/10.1093/infdis/jiaa680
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33128369!7665662!33128369
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suck abstract from ncbi


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pmid33128369      J+Infect+Dis 2021 ; 223 (1): 56-61
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  • Analysis of Humoral Immune Responses in Patients With Severe Acute Respiratory Syndrome Coronavirus 2 Infection #MMPMID33128369
  • Henss L; Scholz T; von Rhein C; Wieters I; Borgans F; Eberhardt FJ; Zacharowski K; Ciesek S; Rohde G; Vehreschild M; Stephan C; Wolf T; Hofmann-Winkler H; Scheiblauer H; Schnierle BS
  • J Infect Dis 2021[Jan]; 223 (1): 56-61 PMID33128369show ga
  • BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has caused a pandemic with tens of millions of cases and hundreds of thousands of deaths. The infection causes coronavirus disease 2019 (COVID-19), a disease of the respiratory system of divergent severity. In the current study, humoral immune responses were characterized in a cohort of 143 patients with COVID-19 from the University Hospital Frankfurt am Main, Germany. METHODS: SARS-CoV-2-specific-antibodies were detected by enzyme-linked immunosorbent assay (ELISA). SARS-CoV-2 and human coronavirus NL63 neutralization activity was analyzed with pseudotyped lentiviral vectors. RESULTS: The severity of COVID-19 increased with age, and male patients encountered more serious symptoms than female patients. Disease severity was correlated with the amount of SARS-CoV-2-specific immunoglobulin (Ig) G and IgA and the neutralization activity of the antibodies. The amount of SARS-CoV-2-specific IgG antibodies decreased with time after polymerase chain reaction conformation of the infection, and antibodies directed against the nucleoprotein waned faster than spike protein-directed antibodies. In contrast, for the common flu coronavirus NL63, COVID-19 disease severity seemed to be correlated with low NL63-neutralizing activities, suggesting the possibility of cross-reactive protection. CONCLUSION: The results describe the humoral immune responses against SARS-CoV-2 and might aid the identification of correlates of protection needed for vaccine development.
  • |*Immunity, Humoral[MESH]
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Antibodies, Neutralizing/immunology[MESH]
  • |Antibodies, Viral/*immunology[MESH]
  • |COVID-19/*immunology[MESH]
  • |Cohort Studies[MESH]
  • |Cross Reactions[MESH]
  • |Enzyme-Linked Immunosorbent Assay[MESH]
  • |Female[MESH]
  • |Germany[MESH]
  • |HEK293 Cells[MESH]
  • |Humans[MESH]
  • |Immunoglobulin A/immunology[MESH]
  • |Immunoglobulin G/immunology[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]


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