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10.1016/j.ijid.2020.10.042 http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.10.042 33127504!7590641!33127504     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Infect+Dis 2021 ; 102 (ä): 181-187 Nephropedia Template TP {{tp|p=33127504|t=2021. Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies |pdf=|usr=}}{{33127504}} gab.com Text Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33127504 #FOAvip OBJECTIVES: To evaluate and compare the performances of five commercial ELISA assays (EDI, AnshLabs, Dia.Pro, NovaTec, and Lionex) for detecting anti-SARS-CoV-2 IgG. METHODS: Seventy negative control samples (collected before the COVID-19 pandemic) and samples from 101 RT-PCR-confirmed SARS-CoV-2 patients (collected at different time points from symptom onset: </=7, 8-14 and >14 days) were used to compare the sensitivity, specificity, agreement, and positive and negative predictive values of each assay with RT-PCR. A concordance assessment between the five assays was also conducted. Cross-reactivity with other HCoV, non-HCoV respiratory viruses, non-respiratory viruses, and nuclear antigens was investigated. RESULTS: Lionex showed the highest specificity (98.6%; 95% CI 92.3-99.8), followed by EDI and Dia.Pro (97.1%; 95% CI 90.2-99.2), NovaTec (85.7%; 95% CI 75.7-92.1), then AnshLabs (75.7%; 95% CI 64.5-84.2). All ELISA kits cross-reacted with one anti-MERS IgG-positive sample, except Lionex. The sensitivity was low during the early stages of the disease but improved over time. After 14 days from symptom onset, Lionex and NovaTec showed the highest sensitivity at 87.9% (95% CI 72.7-95.2) and 86.4% (95% CI 78.5-91.7), respectively. The agreement with RT-PCR results based on Cohen's kappa was as follows: Lionex (0.89) > NovaTec (0.70) > Dia.Pro (0.69) > AnshLabs (0.63) > EDI (0.55). CONCLUSION: The Lionex and NovaLisa IgG ELISA kits, demonstrated the best overall performance. Twit Text FOAVip Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33127504 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33127504 #FOAvip English Wikipedia <ref>{{Cite pmid|33127504}}</ref> Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies #MMPMID33127504 Yassine HM; Al-Jighefee H; Al-Sadeq DW; Dargham SR; Younes SN; Shurrab F; Marei RM; Hssain AA; Taleb S; Alhussain H; Al-Nesf MA; Al-Khal A; Qotba H; Althani AA; Tang P; Abu-Raddad LJ; Nasrallah GK Int J Infect Dis 2021[Jan]; 102 (ä): 181-187 PMID33127504show ga OBJECTIVES: To evaluate and compare the performances of five commercial ELISA assays (EDI, AnshLabs, Dia.Pro, NovaTec, and Lionex) for detecting anti-SARS-CoV-2 IgG. METHODS: Seventy negative control samples (collected before the COVID-19 pandemic) and samples from 101 RT-PCR-confirmed SARS-CoV-2 patients (collected at different time points from symptom onset: 14 days) were used to compare the sensitivity, specificity, agreement, and positive and negative predictive values of each assay with RT-PCR. A concordance assessment between the five assays was also conducted. Cross-reactivity with other HCoV, non-HCoV respiratory viruses, non-respiratory viruses, and nuclear antigens was investigated. RESULTS: Lionex showed the highest specificity (98.6%; 95% CI 92.3-99.8), followed by EDI and Dia.Pro (97.1%; 95% CI 90.2-99.2), NovaTec (85.7%; 95% CI 75.7-92.1), then AnshLabs (75.7%; 95% CI 64.5-84.2). All ELISA kits cross-reacted with one anti-MERS IgG-positive sample, except Lionex. The sensitivity was low during the early stages of the disease but improved over time. After 14 days from symptom onset, Lionex and NovaTec showed the highest sensitivity at 87.9% (95% CI 72.7-95.2) and 86.4% (95% CI 78.5-91.7), respectively. The agreement with RT-PCR results based on Cohen's kappa was as follows: Lionex (0.89) > NovaTec (0.70) > Dia.Pro (0.69) > AnshLabs (0.63) > EDI (0.55). CONCLUSION: The Lionex and NovaLisa IgG ELISA kits, demonstrated the best overall performance. |*Reagent Kits, Diagnostic[MESH] |Adult[MESH] |Antibodies, Viral/*blood[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Cross Reactions[MESH] |Enzyme-Linked Immunosorbent Assay/*methods[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin G/*blood[MESH] |Male[MESH] |Middle Aged[MESH]Performance evaluation of five ELISA kits for detecting anti-SARS-COV-(epmc).pdf DeepDyve Pubget Overpricing