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10.1016/j.bios.2020.112766 http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112766 33126177!7586109!33126177     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33126177&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 172 (ä): 112766 Nephropedia Template TP {{tp|p=33126177|t=2021. opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection |pdf=|usr=}}{{33126177}} gab.com Text opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33126177 #FOAvip The 2019 novel coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected all aspects of human life. Rapid, accurate, sensitive and user friendly detection method is urgently needed to facilitate early intervention and control the spread of SARS-CoV-2. Here, we propose a one-pot visual SARS-CoV-2 detection system named "opvCRISPR" by integrating reverse transcription loop-mediated isothermal amplification (RT-LAMP) and Cas12a cleavage in a single reaction system. We demonstrate that the collateral activity against single-stranded DNA (ssDNA) reporters of activated Cas12a triggered by RT-LAMP amplicon increases detection sensitivity and makes detection results observable with naked eye. The opvCRISPR enables detection at nearly single molecule level in 45 min. We validate this method with 50 SARS-CoV-2 potentially infected clinical samples. The opvCRISPR diagnostic results provide 100% agreement with the Centers for Disease Control and Prevention (CDC)-approved quantitative RT-PCR assay. The opvCRISPR holds great potential for SARS-CoV-2 detection in next-generation point-of-care molecular diagnostics. Twit Text FOAVip opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33126177 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33126177 #FOAvip English Wikipedia <ref>{{Cite pmid|33126177}}</ref> opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection #MMPMID33126177 Wang R; Qian C; Pang Y; Li M; Yang Y; Ma H; Zhao M; Qian F; Yu H; Liu Z; Ni T; Zheng Y; Wang Y Biosens Bioelectron 2021[Jan]; 172 (ä): 112766 PMID33126177show ga The 2019 novel coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected all aspects of human life. Rapid, accurate, sensitive and user friendly detection method is urgently needed to facilitate early intervention and control the spread of SARS-CoV-2. Here, we propose a one-pot visual SARS-CoV-2 detection system named "opvCRISPR" by integrating reverse transcription loop-mediated isothermal amplification (RT-LAMP) and Cas12a cleavage in a single reaction system. We demonstrate that the collateral activity against single-stranded DNA (ssDNA) reporters of activated Cas12a triggered by RT-LAMP amplicon increases detection sensitivity and makes detection results observable with naked eye. The opvCRISPR enables detection at nearly single molecule level in 45 min. We validate this method with 50 SARS-CoV-2 potentially infected clinical samples. The opvCRISPR diagnostic results provide 100% agreement with the Centers for Disease Control and Prevention (CDC)-approved quantitative RT-PCR assay. The opvCRISPR holds great potential for SARS-CoV-2 detection in next-generation point-of-care molecular diagnostics. |*CRISPR-Cas Systems[MESH] |Base Sequence[MESH] |COVID-19 Nucleic Acid Testing/instrumentation/*methods/statistics & numerical data[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/instrumentation/*methods/statistics & numerical data[MESH] |Nucleic Acid Amplification Techniques/instrumentation/*methods/statistics & numerical data[MESH] |Pandemics[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH]opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detec(epmc).pdf DeepDyve Pubget Overpricing