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10.1016/j.jcv.2020.104660 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104660 33126109!7553900!33126109     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2020 ; 133 (ä): 104660 Nephropedia Template TP {{tp|p=33126109|t=2020. Detection of SARS-COV N2 Gene: Very low amounts of viral RNA or false positive?|pdf=|usr=}}{{33126109}} gab.com Text Detection of SARS-COV N2 Gene: Very low amounts of viral RNA or false positive JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33126109 #FOAvip BACKGROUND: The detection of a low amount of viral RNA is crucial to identify a SARS-CoV-2 positive individual harboring a low level of virus, especially during the convalescent period. However, the detection of one gene at high Cycle threshold (Ct) has to be interpreted with caution. In this study we address this specific issue and report our real-life experience. STUDY DESIGN: A total of 1639 nasopharyngeal swabs (NPS) were analyzed with Xpert(R) Xpress SARS-CoV-2. Positive samples showing high Ct values (Ct>35) were concentrated by centrifugation and re-tested with Cepheid or other methods (RealStar SARS-CoV2 RT-PCR, Altona Diagnostics; GeneFinder COVID-19 Plus RealAmp Kit, Elitech). RESULTS: 1599 (97.5%) negative samples, 36 (2.3%) positive samples and 4 (0.2%) presumptive positive samples were detected. In 17 out of 36 positive patients, very low viral RNA copies were suspected since positivity was detected at high Ct. We confirmed positivity for patients who showed both E and N genes detected and for patients with only N detected but with Ct <39. On the contrary, samples with only gene N detected with Ct values >39 were found negative. NPS taken 24 hours after the first collection confirmed the negativity of the 12 samples. Clinical data sustained these results since only 2 of these 12 patients showed COVID-19-like symptoms. CONCLUSIONS: These data support our consideration that detection of the N2 gene at high Ct needs to be interpreted with caution, suggesting that collaboration between virologists and clinicians is important for better understanding of results. Twit Text FOAVip Detection of SARS-COV N2 Gene: Very low amounts of viral RNA or false positive ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33126109 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33126109 #FOAvip English Wikipedia <ref>{{Cite pmid|33126109}}</ref> Detection of SARS-COV N2 Gene: Very low amounts of viral RNA or false positive? #MMPMID33126109 Falasca F; Sciandra I; Di Carlo D; Gentile M; Deales A; Antonelli G; Turriziani O J Clin Virol 2020[Dec]; 133 (ä): 104660 PMID33126109show ga BACKGROUND: The detection of a low amount of viral RNA is crucial to identify a SARS-CoV-2 positive individual harboring a low level of virus, especially during the convalescent period. However, the detection of one gene at high Cycle threshold (Ct) has to be interpreted with caution. In this study we address this specific issue and report our real-life experience. STUDY DESIGN: A total of 1639 nasopharyngeal swabs (NPS) were analyzed with Xpert(R) Xpress SARS-CoV-2. Positive samples showing high Ct values (Ct>35) were concentrated by centrifugation and re-tested with Cepheid or other methods (RealStar SARS-CoV2 RT-PCR, Altona Diagnostics; GeneFinder COVID-19 Plus RealAmp Kit, Elitech). RESULTS: 1599 (97.5%) negative samples, 36 (2.3%) positive samples and 4 (0.2%) presumptive positive samples were detected. In 17 out of 36 positive patients, very low viral RNA copies were suspected since positivity was detected at high Ct. We confirmed positivity for patients who showed both E and N genes detected and for patients with only N detected but with Ct <39. On the contrary, samples with only gene N detected with Ct values >39 were found negative. NPS taken 24 hours after the first collection confirmed the negativity of the 12 samples. Clinical data sustained these results since only 2 of these 12 patients showed COVID-19-like symptoms. CONCLUSIONS: These data support our consideration that detection of the N2 gene at high Ct needs to be interpreted with caution, suggesting that collaboration between virologists and clinicians is important for better understanding of results. |*COVID-19 Nucleic Acid Testing[MESH] |*False Positive Reactions[MESH] |COVID-19/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins/*genetics[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Phosphoproteins/genetics[MESH] |RNA, Viral/*analysis[MESH]Detection of SARS-COV N2 Gene: Very low amounts of viral RNA or false (epmc).pdf DeepDyve Pubget Overpricing