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10.1371/journal.pone.0241542

http://scihub22266oqcxt.onion/10.1371/journal.pone.0241542
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33125422!7598519!33125422
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suck abstract from ncbi


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pmid33125422      PLoS+One 2020 ; 15 (10): e0241542
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  • Sample adequacy controls for infectious disease diagnosis by oral swabbing #MMPMID33125422
  • Deviaene M; Weigel KM; Wood RC; Luabeya AKK; Jones-Engel L; Hatherill M; Cangelosi GA
  • PLoS One 2020[]; 15 (10): e0241542 PMID33125422show ga
  • Oral swabs are emerging as a non-invasive sample type for diagnosing infectious diseases including Ebola, tuberculosis (TB), and COVID-19. To assure proper sample collection, sample adequacy controls (SACs) are needed that detect substances indicative of samples collected within the oral cavity. This study evaluated two candidate SACs for this purpose. One detected representative oral microbiota (Streptococcus species DNA) and the other, human cells (human mitochondrial DNA, mtDNA). Quantitative PCR (qPCR) assays for the two target cell types were applied to buccal swabs (representing samples collected within the oral cavity) and hand swabs (representing improperly collected samples) obtained from 51 healthy U.S. volunteers. Quantification cycle (Cq) cutoffs that maximized Youden's index were established for each assay. The streptococcal target at a Cq cutoff of
  • |Adult[MESH]
  • |COVID-19 Testing/*methods[MESH]
  • |COVID-19/*diagnosis/epidemiology/transmission/virology[MESH]
  • |DNA, Mitochondrial/analysis/genetics[MESH]
  • |DNA, Viral/analysis/genetics[MESH]
  • |Diagnostic Tests, Routine/methods[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Mouth/virology[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |Reference Standards[MESH]
  • |SARS-CoV-2/*isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]
  • |South Africa/epidemiology[MESH]
  • |Specimen Handling/*methods[MESH]


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