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10.3389/fcimb.2020.575613 http://scihub22266oqcxt.onion/10.3389/fcimb.2020.575613 33123498!7572854!33123498     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Cell+Infect+Microbiol 2020 ; 10 (ä): 575613 Nephropedia Template TP {{tp|p=33123498|t=2020. Within-Host Diversity of SARS-CoV-2 in COVID-19 Patients With Variable Disease Severities |pdf=|usr=}}{{33123498}} gab.com Text Within-Host Diversity of SARS-CoV-2 in COVID-19 Patients With Variable Disease Severities JO: Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33123498 #FOAvip Background: The ongoing pandemic of SARS-COV-2 has already infected more than eight million people worldwide. The majority of COVID-19 patients either are asymptomatic or have mild symptoms. Yet, about 15% of the cases experience severe complications and require intensive care. Factors determining disease severity are not yet fully characterized. Aim: Here, we investigated the within-host virus diversity in COVID-19 patients with different clinical manifestations. Methods: We compared SARS-COV-2 genetic diversity in 19 mild and 27 severe cases. Viral RNA was extracted from nasopharyngeal samples and sequenced using the Illumina MiSeq platform. This was followed by deep-sequencing analyses of SARS-CoV-2 genomes at both consensus and sub-consensus sequence levels. Results: Consensus sequences of all viruses were very similar, showing more than 99.8% sequence identity regardless of the disease severity. However, the sub-consensus analysis revealed significant differences in within-host diversity between mild and severe cases. Patients with severe symptoms exhibited a significantly (p-value 0.001) higher number of variants in coding and non-coding regions compared to mild cases. Analysis also revealed higher prevalence of some variants among severe cases. Most importantly, severe cases exhibited significantly higher within-host diversity (mean = 13) compared to mild cases (mean = 6). Further, higher within-host diversity was observed in patients above the age of 60 compared to the younger age group. Conclusion: These observations provided evidence that within-host diversity might play a role in the development of severe disease outcomes in COVID-19 patients; however, further investigations are required to elucidate this association. Twit Text FOAVip Within-Host Diversity of SARS-CoV-2 in COVID-19 Patients With Variable Disease Severities ????Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33123498 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33123498 #FOAvip English Wikipedia <ref>{{Cite pmid|33123498}}</ref> Within-Host Diversity of SARS-CoV-2 in COVID-19 Patients With Variable Disease Severities #MMPMID33123498 Al Khatib HA; Benslimane FM; Elbashir IE; Coyle PV; Al Maslamani MA; Al-Khal A; Al Thani AA; Yassine HM Front Cell Infect Microbiol 2020[]; 10 (ä): 575613 PMID33123498show ga Background: The ongoing pandemic of SARS-COV-2 has already infected more than eight million people worldwide. The majority of COVID-19 patients either are asymptomatic or have mild symptoms. Yet, about 15% of the cases experience severe complications and require intensive care. Factors determining disease severity are not yet fully characterized. Aim: Here, we investigated the within-host virus diversity in COVID-19 patients with different clinical manifestations. Methods: We compared SARS-COV-2 genetic diversity in 19 mild and 27 severe cases. Viral RNA was extracted from nasopharyngeal samples and sequenced using the Illumina MiSeq platform. This was followed by deep-sequencing analyses of SARS-CoV-2 genomes at both consensus and sub-consensus sequence levels. Results: Consensus sequences of all viruses were very similar, showing more than 99.8% sequence identity regardless of the disease severity. However, the sub-consensus analysis revealed significant differences in within-host diversity between mild and severe cases. Patients with severe symptoms exhibited a significantly (p-value 0.001) higher number of variants in coding and non-coding regions compared to mild cases. Analysis also revealed higher prevalence of some variants among severe cases. Most importantly, severe cases exhibited significantly higher within-host diversity (mean = 13) compared to mild cases (mean = 6). Further, higher within-host diversity was observed in patients above the age of 60 compared to the younger age group. Conclusion: These observations provided evidence that within-host diversity might play a role in the development of severe disease outcomes in COVID-19 patients; however, further investigations are required to elucidate this association. |*Severity of Illness Index[MESH] |Adult[MESH] |Aged[MESH] |Betacoronavirus/*classification/*genetics[MESH] |COVID-19[MESH] |Consensus Sequence/genetics[MESH] |Coronavirus Infections/pathology[MESH] |Female[MESH] |Genetic Variation/*genetics[MESH] |Genome, Viral/*genetics[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Pandemics[MESH] |Pneumonia, Viral/pathology[MESH] |RNA, Viral/genetics[MESH] |Risk Factors[MESH] |SARS-CoV-2[MESH] |Sequence Analysis, RNA[MESH]Within-Host Diversity of SARS-CoV-2 in COVID-19 Patients With Variable(epmc).pdf DeepDyve Pubget Overpricing