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10.1016/j.bios.2020.112753

http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112753
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33120235!7575433!33120235
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suck abstract from ncbi


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pmid33120235      Biosens+Bioelectron 2021 ; 171 (ä): 112753
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  • Controlled copper in situ growth-amplified lateral flow sensors for sensitive, reliable, and field-deployable infectious disease diagnostics #MMPMID33120235
  • Zhou Y; Chen Y; Liu Y; Fang H; Huang X; Leng Y; Liu Z; Hou L; Zhang W; Lai W; Xiong Y
  • Biosens Bioelectron 2021[Jan]; 171 (ä): 112753 PMID33120235show ga
  • A polyethyleneimine (PEI)-assisted copper in-situ growth (CISG) strategy was proposed as a controlled signal amplification strategy to enhance the sensitivity of gold nanoparticle-based lateral flow sensors (AuNP-LFS). The controlled signal amplification is achieved by introducing PEI as a structure-directing agent to regulate the thermodynamics of anisotropic Cu nanoshell growth on the AuNP surface, thus controlling shape and size of the resultant AuNP@Cu core-shell nanostructures and confining free reduction and self-nucleation of Cu(2+) for improved reproducibility and decreased false positives. The PEI-CISG-enhanced AuNP-LFS showed ultrahigh sensitivities with the detection limits of 50 fg mL(-1) for HIV-1 capsid p24 antigen and 6 CFU mL(-1) for Escherichia coli O157:H7. We further demonstrated its clinical diagnostic efficacy by configuring PEI-CISG into a commercial AuNP-LFS detection kit for SARS-CoV-2 antibody detection. Altogether, this work provides a reliable signal amplification platform to dramatically enhance the sensitivity of AuNP-LFS for rapid and accurate diagnostics of various infectious diseases.
  • |Betacoronavirus/isolation & purification[MESH]
  • |Biosensing Techniques/instrumentation/*methods[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques[MESH]
  • |Copper/*chemistry[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |Equipment Design[MESH]
  • |Escherichia coli Infections/*diagnosis[MESH]
  • |Escherichia coli O157/isolation & purification[MESH]
  • |Gold/*chemistry[MESH]
  • |HIV Core Protein p24/analysis[MESH]
  • |HIV Infections/*diagnosis[MESH]
  • |HIV-1/isolation & purification[MESH]
  • |Humans[MESH]
  • |Limit of Detection[MESH]
  • |Metal Nanoparticles/chemistry/ultrastructure[MESH]
  • |Oxidation-Reduction[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |Polyethyleneimine/chemistry[MESH]
  • |Reagent Strips/analysis[MESH]


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