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10.1093/jalm/jfaa197

http://scihub22266oqcxt.onion/10.1093/jalm/jfaa197
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33119112!7665556!33119112
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suck abstract from ncbi


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pmid33119112      J+Appl+Lab+Med 2021 ; 6 (3): 606-613
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  • Swab-Free Transport as an Optimized Preanalytical Workflow for SARS-CoV-2 Amplification #MMPMID33119112
  • Greene DN; Matthys T; Lockwood CM
  • J Appl Lab Med 2021[Apr]; 6 (3): 606-613 PMID33119112show ga
  • INTRODUCTION: Efficient detection of SARS-CoV-2 will continue to be an invaluable tool for pandemic control. Current instructions specify that the collection swab should be transported within its collection media to the laboratory. Developing a process whereby this swab is removed before transport to the lab would allow for improved automation and decreased manual manipulation of samples. METHODS: A proof of principle approach was taken by eluting viral particles from flocked swabs into collection buffer with and without a mucus background. Paired swab-free and swab-containing samples were transported to the laboratory and evaluated for SARS-CoV-2 (n = 28) or RNaseP (n = 6). SARS-CoV-2 amplification was performed using the Hologic Panther Fusion Aptima and RT-PCR assays. RESULTS: SARS-CoV-2 was detected in all proof of principle samples with Ct values indicative of dilution. The rare exception was for a few samples where the dilution pushed the viral load below the LOD. Paired samples were 100% concordant for SARS-CoV-2 and RNaseP detection. CONCLUSION: Discarding the swab after inoculating the transport buffer is an appropriate preanalytical modification. Adopting this approach can save up to 1 minute per sample. For labs processing more than 500 samples per day this equates to 1 full time equivalent shift per day.
  • |*Viral Load[MESH]
  • |*Workflow[MESH]
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Limit of Detection[MESH]
  • |Proof of Concept Study[MESH]
  • |Ribonuclease P/analysis[MESH]
  • |SARS-CoV-2[MESH]


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