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10.3390/v12111214 http://scihub22266oqcxt.onion/10.3390/v12111214 33114742!7692607!33114742     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (11): ä Nephropedia Template TP {{tp|p=33114742|t=2020. Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization |pdf=|usr=}}{{33114742}} gab.com Text Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33114742 #FOAvip Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals, as well as S-specific monoclonal antibodies, were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to the binding of S glycoprotein in the context of viral particles remains to be established. Here, we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA, ELISA, and neutralization assays, we observed a strong correlation between these parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of pseudoviral particles is required but not sufficient to mediate neutralization. Altogether, our results highlight the importance of better understanding the inactivation of S by plasma and neutralizing antibodies. Twit Text FOAVip Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33114742 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33114742 #FOAvip English Wikipedia <ref>{{Cite pmid|33114742}}</ref> Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization #MMPMID33114742 Ding S; Laumaea A; Benlarbi M; Beaudoin-Bussieres G; Gasser R; Medjahed H; Pancera M; Stamatatos L; McGuire AT; Bazin R; Finzi A Viruses 2020[Oct]; 12 (11): ä PMID33114742show ga Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals, as well as S-specific monoclonal antibodies, were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to the binding of S glycoprotein in the context of viral particles remains to be established. Here, we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA, ELISA, and neutralization assays, we observed a strong correlation between these parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of pseudoviral particles is required but not sufficient to mediate neutralization. Altogether, our results highlight the importance of better understanding the inactivation of S by plasma and neutralizing antibodies. |Animals[MESH] |Antibodies, Immobilized/immunology[MESH] |Antibodies, Monoclonal/immunology[MESH] |Antibodies, Neutralizing/immunology[MESH] |Antibodies, Viral/*immunology[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |Cell Line[MESH] |Convalescence[MESH] |Coronavirus Infections/*immunology[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Neutralization Tests[MESH] |Pandemics[MESH] |Pneumonia, Viral/*immunology[MESH] |SARS-CoV-2[MESH] |Spike Glycoprotein, Coronavirus/*immunology[MESH]Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does(epmc).pdf DeepDyve Pubget Overpricing