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10.1016/j.bios.2020.112685

http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112685
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33113383!7557276!33113383
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suck abstract from ncbi


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pmid33113383      Biosens+Bioelectron 2021 ; 171 (ä): 112685
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  • One-step rapid quantification of SARS-CoV-2 virus particles via low-cost nanoplasmonic sensors in generic microplate reader and point-of-care device #MMPMID33113383
  • Huang L; Ding L; Zhou J; Chen S; Chen F; Zhao C; Xu J; Hu W; Ji J; Xu H; Liu GL
  • Biosens Bioelectron 2021[Jan]; 171 (ä): 112685 PMID33113383show ga
  • The spread of SARS-CoV-2 virus in the ongoing global pandemic has led to infections of millions of people and losses of many lives. The rapid, accurate and convenient SARS-CoV-2 virus detection is crucial for controlling and stopping the pandemic. Diagnosis of patients in the early stage infection are so far limited to viral nucleic acid or antigen detection in human nasopharyngeal swab or saliva samples. Here we developed a method for rapid and direct optical measurement of SARS-CoV-2 virus particles in one step nearly without any sample preparation using a spike protein specific nanoplasmonic resonance sensor. As low as 370 vp/mL were detected in one step within 15 min and the virus concentration can be quantified linearly in the range of 0 to 10(7) vp/mL. Measurements shown on both generic microplate reader and a handheld smartphone connected device suggest that our low-cost and rapid detection method may be adopted quickly under both regular clinical environment and resource-limited settings.
  • |*Clinical Laboratory Techniques/economics[MESH]
  • |*Point-of-Care Testing[MESH]
  • |Antibodies, Immobilized/chemistry[MESH]
  • |Betacoronavirus/*isolation & purification[MESH]
  • |Biosensing Techniques/economics/*instrumentation[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Coronavirus Infections/*diagnosis/economics[MESH]
  • |Equipment Design[MESH]
  • |Humans[MESH]
  • |Limit of Detection[MESH]
  • |Models, Molecular[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |SARS-CoV-2[MESH]
  • |Spike Glycoprotein, Coronavirus/analysis[MESH]
  • |Time Factors[MESH]


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