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10.1590/0074-02760200342 http://scihub22266oqcxt.onion/10.1590/0074-02760200342 33111751!7586445!33111751     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mem+Inst+Oswaldo+Cruz 2020 ; 115 (ä): e200342 Nephropedia Template TP {{tp|p=33111751|t=2020. SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network |pdf=|usr=}}{{33111751}} gab.com Text SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network JO: Mem Inst Oswaldo Cruz http://www.kidney.de/pget.php?&tw=1&pmid=33111751 #FOAvip BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES: The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS: After diagnosis in patients that returned from Italy to the Sao Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS: The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 x 106 TCID50/mL, titre 1.5 x 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION: We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research. Twit Text FOAVip SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network ????Mem Inst Oswaldo Cruz http://www.kidney.de/pget.php?&tw=1&pmid=33111751 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33111751 #FOAvip English Wikipedia <ref>{{Cite pmid|33111751}}</ref> SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network #MMPMID33111751 Araujo DB; Machado RRG; Amgarten DE; Malta FM; de Araujo GG; Monteiro CO; Candido ED; Soares CP; de Menezes FG; Pires ACC; Santana RAF; Viana AO; Dorlass E; Thomazelli L; Ferreira LCS; Botosso VF; Carvalho CRG; Oliveira DBL; Pinho JRR; Durigon EL Mem Inst Oswaldo Cruz 2020[]; 115 (ä): e200342 PMID33111751show ga BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES: The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS: After diagnosis in patients that returned from Italy to the Sao Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS: The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 x 106 TCID50/mL, titre 1.5 x 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION: We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research. |*Coronavirus Infections[MESH] |*Pandemics[MESH] |*Pneumonia, Viral[MESH] |Animals[MESH] |Betacoronavirus/*isolation & purification[MESH] |Brazil[MESH] |COVID-19[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |SARS-CoV-2[MESH]SARS-CoV-2 isolation from the first reported patients in Brazil and es(epmc).pdf DeepDyve Pubget Overpricing