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10.1039/d0ay01661f http://scihub22266oqcxt.onion/10.1039/d0ay01661f 33111715!ä!33111715 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Methods 2020 ; 12 (44): 5392-5396 Nephropedia Template TP {{tp|p=33111715|t=2020. Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids |pdf=|usr=}}{{33111715}} gab.com Text Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids JO: Anal Methods http://www.kidney.de/pget.php?&tw=1&pmid=33111715 #FOAvip Multiplex PCR encounters difficulties in primer designing with all the primer pairs working at the same annealing temperature. In this study, we have developed a double-strand primer-mediated multiple strand displacement reaction for the detection of SARS-COV-2 ORF, N and E genes (as examples). The double primer is composed of a 5'-modified fluorophore strand, which does not impact polymerase extension and a 3'-modified quencher strand, which cannot impact elongation. At the annealing temperature, the fluorophore strand combined with the template, extended and resulted in fluorescence signal release. Results showed that the double-strand primer relatively exhibits a wide annealing temperature range and good compatibility between three pairs of primers and probes. These merits allow the simple and multiplex real-time fluorescence quantification of nucleic acids. The detection limit was 400 copies/mL, and the detection time was approximately 2 h. In addition to its extreme specificity and simplicity, this method has a wide range of applications such as multiple PCR and SNP detection. Twit Text FOAVip Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids ????Anal Methods http://www.kidney.de/pget.php?&tw=1&pmid=33111715 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33111715 #FOAvip English Wikipedia <ref>{{Cite pmid|33111715}}</ref> Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids #MMPMID33111715 Zhang Z; Yao J; Huang X; Zhang L; Wang T; Weng Z; Xie G Anal Methods 2020[Nov]; 12 (44): 5392-5396 PMID33111715show ga Multiplex PCR encounters difficulties in primer designing with all the primer pairs working at the same annealing temperature. In this study, we have developed a double-strand primer-mediated multiple strand displacement reaction for the detection of SARS-COV-2 ORF, N and E genes (as examples). The double primer is composed of a 5'-modified fluorophore strand, which does not impact polymerase extension and a 3'-modified quencher strand, which cannot impact elongation. At the annealing temperature, the fluorophore strand combined with the template, extended and resulted in fluorescence signal release. Results showed that the double-strand primer relatively exhibits a wide annealing temperature range and good compatibility between three pairs of primers and probes. These merits allow the simple and multiplex real-time fluorescence quantification of nucleic acids. The detection limit was 400 copies/mL, and the detection time was approximately 2 h. In addition to its extreme specificity and simplicity, this method has a wide range of applications such as multiple PCR and SNP detection. |*SARS-CoV-2/chemistry[MESH] |COVID-19 Nucleic Acid Testing/methods[MESH] |COVID-19/diagnosis[MESH] |Coronavirus Envelope Proteins/*genetics[MESH] |Coronavirus Nucleocapsid Proteins/*genetics[MESH] |DNA Primers/chemistry/genetics[MESH] |DNA, Viral/*analysis[MESH] |Humans[MESH] |Limit of Detection[MESH] |Multiplex Polymerase Chain Reaction/*methods[MESH] |Phosphoproteins/genetics[MESH]Multiplex real-time PCR using double-strand primers and probes for the(epmc).pdf DeepDyve Pubget Overpricing